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Actinomycetes with broad-spectrum antibacterial activity and screening method thereof

A technology of antibacterial activity and actinomycetes, applied in the field of microorganisms, can solve the problems of unfavorable pollution-free green fruit production, unsatisfactory control effect, and polluted soil environment, and achieve pollution-free, significant control effect, and no soil pollution. Effect

Inactive Publication Date: 2016-05-04
BEIJING UNIV OF AGRI
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  • Claims
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Problems solved by technology

[0004] However, at present, these methods of controlling plant diseases with chemical pesticides have unsatisfactory control effects, and the use of chemical pesticides to prevent and control plant diseases will pollute the soil environment, which is not conducive to pollution-free and green fruit production

Method used

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  • Actinomycetes with broad-spectrum antibacterial activity and screening method thereof
  • Actinomycetes with broad-spectrum antibacterial activity and screening method thereof
  • Actinomycetes with broad-spectrum antibacterial activity and screening method thereof

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Embodiment Construction

[0013] The present invention will be described in further detail below through specific implementation examples and in conjunction with the accompanying drawings.

[0014] An actinomycete with broad-spectrum antibacterial activity provided by Example 1 of the present invention, the bacterium is Streptomycessp., has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on March 5, 2013, and the depository unit The address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences; the preservation number is CGMCC No. 7278; its classification name is: Streptomyces sp. According to the experimental data, Streptomycesssp. is effective against plum dry rot pathogen Polvporushirsutus, gray mold pathogen Botrytiscinerea Phomopsisvexans, Rhizopus nigricans, potato dry rot pathogen Fusariumcaerulem (Libert) Sacc., cabbage black spot fungus Alternariabrassicae, ...

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Abstract

The invention relates to the technical field of microorganisms, and specifically, particularly relates to actinomycetes with broad-spectrum antimicrobial activity and a screening method thereof. The actinomycetes with broad-spectrum antimicrobial activity belong to Streptomyces sp, and the preservation number is CGMCC No.7278. The screening method of the actinomycetes with broad-spectrum antimicrobial activity comprises the following steps: obtaining cultivated streptomyces from a to-be-separated soil sample; screening to obtain actinomycetes of antagonism target bacteria from the cultivated actinomycetes by using a plate confrontation method, an inhibition zone method or a cross patch method. By adopting the actinomycetes with broad-spectrum antimicrobial activity and the screening method thereof provided by the invention, the prevention role on the plant disease is played by inhibiting the growth and propagation action of the pathogenic bacteria. Compared with the traditional chemical pesticide actinomycetes for preventing and treating the plant disease, the method is significant in prevention and treatment effects, free of pollution to soil, and beneficial to production of pollution-free green products.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to actinomycetes with broad-spectrum antibacterial activity and a screening method thereof. Background technique [0002] Plant diseases are caused by a variety of pathogenic bacteria. For example, peach tree root cancer (also known as crown gall) is a worldwide plant bacterial tumor disease caused by Agrobacterium tumefaciens. Peach trees all over my country It occurs in all cultivation areas, and in some areas, the incidence rate is as high as 100%. Once the plants are infected, it is difficult to cure, ranging from weak yellow leaves to dead trees in severe cases; plum rot is an important disease that harms branches and fruits. It is an important reason for the low survival rate of plum planting and the death of saplings. The pathogen is Polvporushirsutus. In addition to harming plum trees, it also harms pears, citrus, drupe fruit trees, some dry fruit tree species, and popl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00C12Q1/04C12R1/465
Inventor 刘志民马焕普薛红燕
Owner BEIJING UNIV OF AGRI
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