A kind of crop pathogen antagonistic actinomycetes and its screening and application

A technology of actinomycetes and pathogens, applied in the field of microorganisms, to achieve the effects of pollution-free, significant control effects, and no soil pollution

Inactive Publication Date: 2019-04-02
HECHI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on the prevention and control of plant diseases and the screening of antagonistic bacteria produced by Fusarium chlamydoides

Method used

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  • A kind of crop pathogen antagonistic actinomycetes and its screening and application
  • A kind of crop pathogen antagonistic actinomycetes and its screening and application
  • A kind of crop pathogen antagonistic actinomycetes and its screening and application

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Embodiment Construction

[0031] All features disclosed in this specification, or all disclosed steps in a method or process, may be combined in any way except mutually exclusive features and / or steps.

[0032] Any feature disclosed in this specification (including any accompanying claims, abstract), unless expressly stated otherwise, may be replaced by other equivalent or alternative features serving a similar purpose. That is, unless expressly stated otherwise, each feature is but one example of a series of equivalent or similar features.

[0033] Isolation and Screening of Strain RS15

[0034] The culture medium used in this example includes the following:

[0035] (1) Gao's No. 1 medium:

[0036] Soluble starch 20.0 g, KNO3 1.0 g, NaCl 0.5 g, K2HPO4 0.5 g, MgSO ·7H2O 0.5g, FeSO4·7H2O 0.01g, agar 20.0g, distilled water 1000mL, pH 7.2~7.4.

[0037] (2) Actinomycetes liquid fermentation medium:

[0038] Soluble starch 5.0 g, glucose 1.0 g, peptone 0.2 g, yeast extract 0.2 g, NaCl 0.2 g, MgSO ·...

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Abstract

The invention belongs to the technical field of microorganisms, and discloses a crop pathogen antagonistic actinomycete and its screening and application. The crop pathogen antagonistic actinomycete belongs to the genus Streptomyces lydida, and the preservation number is: CCTCC M 2016038. The screening method includes: (1) adopting the gradient dilution method to obtain cultured actinomycetes from soil samples; (2) using the agar block method to obtain actinomycetes that antagonize the target bacteria; (3) using the actinomycetes to Bacteria were cultured at constant temperature to obtain actinomycete fermentation liquid; (4) the target bacteria were made into a PDA plate containing bacteria, and the diameter of the inhibition zone was measured by the Oxford cup method; (5) the actinomycetes whose diameter of the inhibition zone was greater than 20mm were selected . The invention prevents and controls plant diseases by inhibiting the growth and reproduction of pathogenic bacteria. Compared with the control of plant diseases by chemical pesticides, the method has significant effect, no pollution to soil, and is beneficial to the production of pollution-free and green products.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a crop pathogen antagonizing actinomycete and its screening and application. Background technique [0002] Agricultural antibiotics refer to secondary metabolites produced by microbial fermentation, with the function of pesticides, and used in agriculture to control pests, weeds, rats and other harmful organisms. According to the function, it can be divided into bactericidal antibiotics, herbicidal antibiotics and insecticidal antibiotics. The application and development of agricultural antibiotics can be traced back to the 1940s. They were initially developed in developed countries with the use of medical antibiotics, such as streptomycin, griseofulvin, etc., but due to their performance and cost, they are comparable to chemical pesticides. Compared with, there is no advantage, so it has been developing slowly. After the 1950s, researchers from various count...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02A01N63/00A01P1/00A01P3/00C12R1/465
CPCA01N63/00C12N1/02C12N1/205C12R2001/465
Inventor 覃拥灵何海燕卢福芝
Owner HECHI UNIV
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