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Method for preparing geniposide in eucommia leaf

A technology of geniposide and Eucommia ulmoides leaves is applied in the field of preparing geniposide from Eucommia ulmoides leaves, which can solve the problems of large amount of waste water, low product purity, low extraction rate and the like, and achieves low water extraction temperature and simple membrane operation process. , the effect of shortening the extraction time

Inactive Publication Date: 2014-05-14
HUBEI LAOLONGDONG DUZHONG DEV
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Problems solved by technology

[0004] In order to overcome the problems and shortcomings in the above-mentioned prior art, the present invention provides a method for preparing geniposide from Eucommia leaves. Extract geniposide from Eucommia ulmoides leaves, then separate and purify the extracted geniposide through two-stage membrane separation technology combined with resin adsorption elution process, collect ethanol eluate, concentrate under reduced pressure, and vacuum dry to obtain the product geniposide Geniposide, which effectively solved the problems of low extraction rate, large amount of waste water and low product purity in the original production of geniposide

Method used

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  • Method for preparing geniposide in eucommia leaf

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Embodiment Construction

[0024] Example 1, see the attached figure 1 , take 10Kg of dried Eucommia leaves, crush them and pass through 80 mesh sieve, add 120Kg of water, adjust the temperature to 55°C, keep the temperature constant and adjust the pH value to 4.5, then add 10g of cellulase, extract for 60min under this condition, filter, and filter the residue again Extract for the second time according to the aforementioned extraction conditions, combine the two filtrates, and filter conventionally, the extraction rate of geniposide is 95.8%; the operating conditions are P=0.3Mpa, T=45°C, PH=4.5, Q=70L / min The filtrate is passed through a 0.03μm ultrafiltration membrane, and the extract is concentrated by 11.2 times under this condition. The average flux of the ultrafiltration membrane is 226.3L / m 2 h; under the operating conditions P=1.5Mpa, T=50°C, PH=4.5, Q=20L / min, the ultrafiltration filtrate is passed through a 200 molecular weight nanofiltration membrane, and the ultrafiltration filtrate is con...

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Abstract

The invention discloses a method for preparing geniposide in eucommia leaf. The method comprises the following steps of crushing eucommia leaf, carrying out cellulase-assistant middle temperature-water extraction, carrying out routine filtration, carrying out ultrafilter membrane impurity-removal, carrying out nanofiltration condensation, carrying out macroporous resin-based separation purification, carrying out condensation and carrying out drying to obtain geniposide powder. The method utilizes cellulase-assistant middle temperature-water extraction of geniposide in eucommia leaf, has an extraction rate of 95%, a low water extraction temperature and short extraction time, utilizes an ultrafilter membrane to remove impurities and separate other side products in the extract, has simple membrane processes and realizes long-term membrane flux stabilization, easy membrane cleaning, a long service life and industrialization. Through the nanofiltration membrane, the extract subjected to impurity removal is concentrate d so that follow-up technical load is reduced and a resin service life is prolonged. Through the method, a nanofiltrated liquid has a COD value <=100 and can be reused in production and basically, waste water is not produced. Through the macroporous adsorption resin, geniposide can be effectively purified. The powder produced by the method has geniposide purity greater than 80% and an extraction rate more than 1.2%.

Description

technical field [0001] The invention relates to a method for preparing geniposide from Eucommia ulmoides leaves, in particular to a method for preparing geniposide by adopting an enzymatic medium-warm water extraction, membrane separation technology and resin technology. Background technique [0002] Geniposide is an iridoid glucoside that is easily soluble in water. Geniposide can be hydrolyzed to genipin by β-glucosidase. Modern studies have shown that geniposide has significant curative effects on digestive system, cardiovascular system and central nervous system diseases. In addition, geniposide also has certain anti-inflammatory and soft tissue injury effects. In addition to medicinal use, geniposide is also widely used in other fields, such as plant yield-increasing agents, biological detection agents, etc., and is an important biologically active substance. It is widely used in the field of medical treatment and health care products. Geniposide is mainly found in n...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08
Inventor 刘冬成任治军刘芸芳
Owner HUBEI LAOLONGDONG DUZHONG DEV
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