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A kind of indica-japonica hybrid rice anther culture method

A hybrid rice and culture method technology, applied in the fields of indica-japonica hybrid rice breeding, molecular biology research and genetic engineering research, can solve the problems of low green seedling differentiation rate, low callus induction rate, hindering the application of flower cultivation technology, etc. , to achieve the effects of enriching genotypes, fast and effective screening of restorer lines, and improving breeding efficiency

Active Publication Date: 2015-12-02
ZHEJIANG NONGKE SEED IND
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Problems solved by technology

However, there are few studies on the anther culture of indica-japonica hybrid rice, and the callus induction rate is low (only about 30%), and the green seedling differentiation rate is low (only about 20%)
This seriously hinders the application of flowering culture technology in the practice of indica and japonica hybrid rice breeding

Method used

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  • A kind of indica-japonica hybrid rice anther culture method
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  • A kind of indica-japonica hybrid rice anther culture method

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Embodiment Construction

[0035] Haploid and double haploid are of great significance and broad application prospects in crop genetic research and breeding practice. The ways of obtaining haploid materials include natural occurrence and artificial induction. The frequency of the former is extremely low, and the latter is most widely used in anther culture. Anther culture is to culture anthers isolated from flower organs as explants in vitro, induce microspore cells to undergo in vitro androgenesis, form embryogenic callus, and then obtain haploid or double haploid after redifferentiation A method of the body. Haploid or double haploid breeding materials of various genotypes of indica-japonica hybrid rice can be quickly obtained by using anther culture technology in indica-japonica hybrid rice breeding, and gene mutations are more likely to occur to obtain new germplasm. We have obtained haploid and double haploid rice breeding materials by applying the method of the present invention.

[0036]In thi...

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Abstract

The invention discloses an anther culture method for indica japonica hybrid rice. The method comprises the following steps: sampling, identifying a microspore development period, low-temperature pretreatment, young wheatear sterilization, anther inoculation, still dark cultivation at 25+ / - 2 DEG C until the diameter of embryonic callus is 2-3mm; carrying out a redifferentiation stage to form complete regenerated plants with haploid or double haploids. The double haploids are good breeding materials, and can be directly used for breeding of new varieties or varietal improvement; the breeding age limit is greatly shortened; the selection efficiency is improved; the double haploids also are ideal materials for molecular markers, such as amplified fragment length polymorphism (AFLP), restricted fragment length polymorphisms (RFLP), randomly amplified polymorphic deoxyribonucleic acid (RAPD) and the like, genetic map drawing, and researches on animal evolution, genetic analysis, plant gene cloning and screening, genetic engineering breeding and the like.

Description

technical field [0001] The invention relates to an indica-japonica hybrid rice anther culture method, which is mainly used for indica-japonica hybrid rice breeding, molecular biology research and genetic engineering research. Background technique [0002] Rice anther culture technology plays an important role in rice breeding. The technology is to inoculate anthers that have developed to a certain stage on an artificial medium, and culture them in vitro under certain light and temperature conditions to induce microspores to form calluses and then differentiate into complete haploid regenerated plants. Afterwards, double haploid plants (DH plants) were generated by artificial doubling or natural doubling. They are genetically absolutely homozygous. In the practice of rice breeding, the use of anther culture can quickly obtain pure lines, greatly shorten the breeding cycle, improve selection efficiency, and reduce breeding costs. In the theoretical research of genetic breed...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 詹艳余守武阮关海阮晓丽陈珊宇黄益峰闫川曹栋栋洪晓富陈合云
Owner ZHEJIANG NONGKE SEED IND
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