Novel lactic acid bacteria and method for preparing silage or fermented feed using same

Abstract

An object of the present invention is to provide a lactic acid bacterium capable of stably producing silage or fermented feed material that is subjected to butyric acid fermentation due to low sugar content and which cannot obtain sufficient fermentation quality improvement effects with conventional lactic acid bacteria. High quality silage or fermented feed. The present invention provides a lactic acid bacterium that produces 40 IU or more of nisin per 1 mL of a supernatant in a pasture juice culture medium containing 0.1 to 0.4 mass % of monosaccharides and disaccharides in total, and a microbial preparation for preparing silage containing the lactic acid bacterium , and a method for preparing silage or fermented feed using the preparation.

Description

technical field [0001] The invention relates to a lactic acid bacterium capable of inhibiting butyric acid fermentation, a microbial preparation containing the lactic acid bacteria and a method for preparing silage or fermented feed using the preparation. Background technique [0002] The silage obtained by lactic acid fermentation of pasture and forage crops, which are the staple food of livestock, under anaerobic conditions is a necessary feed in today's dairy industry. The preparation of high-quality silage is a very important technology for dairy farmers. At this time, a problem called poor fermentation occurs frequently. This is a problem that butyric acid bacteria are more advantageous than lactic acid bacteria in silage, and as a result, the butyric acid content in silage becomes high. Silage with a high concentration of butyric acid not only causes a reduction in appetite and feed intake of cattle, but also causes problems related to diseases such as ketosis. Theref...

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Problems solved by technology

Therefore, lactic acid bacteria producing high concentrations of nisin in a synthetic medium and nisin producing in a fermented barley extract medium have also been reported (Patent Document 1, Non-Patent Document 6), but no synthetic Existing literature on the production of high concentrations of nisin in culture medium or fermented barley extract medium, and in silage materials with different nutrient sources

[0007] In addition, in the case of silage as an object, a silage preparation method in which bacteriocin-producing Lactococcus lactis RO50 strain is added to silage material grass has been reported (Patent Document 2), but it has also been reported that sufficient silage is not obtained. In the case of improving the fermentation quality, the effect is not stable, and it is completely unclear whether bacteriocin is produced in the silage (Non-Patent Documents 7 and 8)

In addition, in Patent Document 3, there is also a report on a method for preparing silage by adding nisin-producing lactic acid bacteria and nisin-resistant lactic acid bacteria. Although 200 IU / g of nisin activity was detected in the silage, but Since there is no change in the fermentation quality such as pH, organic acid content, and ammoniacal nitrogen concentration relative to total nitrogen compared with other lactic acid bacteria that do not produce nisin, good fermentation can be obtained even with common lactic acid bacteria The results of the sugar content (5.0% or more in the dry product, 1.0% or more in the raw material) are not yet clear about the effect of this lactic acid bacteria under the strict conditions of low sugar content

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