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A siRNA that interferes with hip-55 and its application in anti-tumor

A HIP-55, tumor technology, applied in anti-tumor drugs, DNA/RNA fragments, applications, etc., can solve problems such as no effect research

Inactive Publication Date: 2016-01-06
PEKING UNIV THIRD HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its role in tumors has not been studied

Method used

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  • A siRNA that interferes with hip-55 and its application in anti-tumor
  • A siRNA that interferes with hip-55 and its application in anti-tumor
  • A siRNA that interferes with hip-55 and its application in anti-tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1, Construction of A549 cells overexpressing HIP-55 and A549 cells knocking down HIP-55

[0042] 1. Obtaining HIP-55siRNA

[0043] The following siRNA sequences were designed and synthesized for the human HIP-55 protein coding gene:

[0044] HIP-55siRNA, its nucleotide sequence is 5'-GAUCCGCAGUGAACGUAGAGAAUUGUUCAAGAGACAAUUCUCUACGUUCACUGUUUUUUGGAAA-3' (SEQ ID NO: 3);

[0045] The nucleotide sequence of the control ScramblesiRNA is 5'-GAUCCGCACUACCGGUUGUAUAGGUGUUCAAGAGACACCUAUACAAAGGUAGUGUUUUGGAAA-3' (SEQ ID NO: 4).

[0046] 2. Construction of recombinant vectors for overexpressing HIP-55 and recombinant vectors for RNA interference HIP-55

[0047] 1. Construction of recombinant vector pLenti6 / V5-HIP-55 overexpressing HIP-55

[0048] Using the gene of HIP-55 shown in sequence 1 in the above sequence listing as a template, use

[0049] Forward: '-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGCGGCGAACCTGAGCC-3' and

[0050] Reverse: 5'-GGGGACCACTTTGTACAAGAAAGCTGGGTCCTCAAT...

Embodiment 2

[0074] Example 2, Functional Research of HIP-55

[0075] 1. Effect of HIP-55 on the proliferation and apoptosis of A549 cells

[0076] 1. The effect of HIP-55 on the proliferation of lung cancer A549 cells

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Abstract

The invention discloses siRNA interfering HIP-55 and anti-tumor application of siRNA interfering HIP-55. The nucleotide sequence of the siRNA is shown in a sequence III in a sequence table. The invention also provides a DNA molecule encoding the above siRNA. Experiments prove that HIP-55 is highly expressed in a tissue sample of a clinical lung cancer patient; and further researches show the following functions of the HIP-55: through interfering silent protein HIP-55 by RNA, tumor formation can be obviously inhibited, apoptosis is promoted, proliferation, cell cloning formation, cell migration and / or cell invasion are suppressed; therefore, siRNA interfering HIP-55 can be used for preparing products for preventing and / or treating tumors.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a siRNA interfering with HIP-55 and its application in anti-tumor. Background technique [0002] HIP-55 (hematopoietic progenitorkinase 1 [HPK1]-interacting protein of 55kDa; also known as drebrin-likeprotein DBNL, mAbp1 or SH3P7) is a protein containing a multifunctional domain. Its structure mainly includes the N-terminal F-actin protein binding domain and the C-terminal SH3 domain. HIP-55 is involved in synaptogenesis, receptor endocytosis, cytoskeleton rearrangement and signal transduction. At present, the function of HIP-55 is poorly understood. It is known that HIP-55 plays an important role in the immune system by regulating T cell receptor endocytosis, cytokine release and B cell receptor presentation. Recent studies have shown that HIP-55 regulates cytoskeletal rearrangements in the nervous system by interacting with N-WASP and Arp2 / 3. However, its role in tumors has not ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/861C12N5/10A61K48/00A61P35/00
Inventor 李子健付海安石智李增刚
Owner PEKING UNIV THIRD HOSPITAL
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