Method for promoting formation of bacillus subtilis spores by using metal ions

Abstract

The invention discloses a method for promoting formation of bacillus subtilis spores by using metal ions. The method comprises the following steps: (1) preparing a culture medium, adding 0.009-0.02% of MnSO4 into the culture medium, preparing a flat plate, and preserving the flat plate at a temperature of 4 DEG C for later use; (2) preserving bacillus subtilis strains in the culture medium, partitioning the flat plate in a zigzag mode, and culturing the strains overnight at a temperature of 37 DEG C; (3) activating the bacillus subtilis strains subjected to overnight culturing; (4) inoculating a seed solution of the bacillus subtilis strains subjected to activating to soybean meal and cottonseeds, and carrying out solid-state fermentation; and (5) drying the fermented product until the moisture content is less than or equal to 10-13%, and crushing so as to obtain a protein feed rich in bioactive peptide. According to the method disclosed by the invention, the fermentation period is shortened. Conditions for producing bacillus subtilis by fermenting are optimized, and fermentation temperature, pH value, rotating speed and liquid filling amount are subjected to condition optimization, so that a fermentation condition with the optimal effect is obtained, the effect of biomass of fermentation can be significantly improved, and therefore, the method is suitable for large-scale fermentation production.

Description

technical field [0001] The invention belongs to the field of microbial fermentation in bioengineering, and more specifically relates to a method for promoting the formation of bacillus subtilis spores by using metal ions, which can significantly increase the biomass of subtilis spores. Background technique [0002] The degradation of pastures and the requirements of the ecological environment have promoted the development of livestock breeding methods, which limit the animals' contact with the soil and the surrounding natural environment. This feeding condition is not conducive to the establishment of normal flora in the gastrointestinal tract of animals. However, when the external conditions change, it is easy to cause the imbalance of micro-ecological flora in the gastrointestinal tract of animals, thereby reducing the resistance to pathogenic bacteria. At present, this loss is mainly reduced by improving feeding management and adding microorganisms to the feed. However, w...

AI Technical Summary

Problems solved by technology

[0007] At present, many domestic Bacillus subtilis production enterprises generally have the problem of low bacterial count, especially low spore rate in the production process. Generally, the spore content is below 2.0×109CFU / ml, which greatly affects the cost and quality of products.