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A cryopreservation device applying electromagnetic freezing method and its operation method

A technology of cryopreservation and freezing, which is applied in the direction of household refrigeration equipment, preservation and application of human or animal bodies, and can solve the problems of affecting the integrity and timeliness of preserved organisms, increasing the cooling speed too quickly, and destroying organisms. Cell structure and other issues, to achieve the effect of improving the quality of protection, fast cooling rate, and ensuring timeliness

Inactive Publication Date: 2016-07-06
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So not only does this approach lead to the formation of larger ice crystals that damage the cellular structure of the organism, but it is also toxic to the cells
Seriously affects the integrity and timeliness of preserved organisms
Although the vitrification method can avoid the cell damage caused by ice crystals to a large extent by adding a cryoprotectant under rapid freezing, the cryoprotectant has a certain degree of damage, and the actual process cannot increase the cooling rate too much. quick

Method used

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  • A cryopreservation device applying electromagnetic freezing method and its operation method
  • A cryopreservation device applying electromagnetic freezing method and its operation method
  • A cryopreservation device applying electromagnetic freezing method and its operation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Such as figure 1 As shown, a cryopreservation device using the electromagnetic freezing method includes a low-temperature constant temperature tank 1, a Helmholtz coil 2, a stabilized power supply 4, and a cooling platform 5; wherein, the low-temperature constant temperature tank 1 is connected to the cooling platform 5 through a circulation pump, The Helmholtz coil 2 is arranged on both sides of the cooling table 5, and the stabilized power supply 4 is connected to the Helmholtz coil 2. The cooling table 5 is made of aluminum alloy with a thermal conductivity of 228W / (m·K). The cooling table 5 A groove 8 is provided on the top, and the vial 3 containing medicine or organism is placed in the groove 8 of the cooling table 5 .

[0026] Such as figure 2 , image 3 As shown, the inside of the cooling platform 5 is provided with a bent cooling liquid flow channel 9, the cooling platform is provided with a cooling liquid inlet 6 and a cooling liquid outlet 7, and the cooli...

Embodiment 2

[0035] Such as figure 1 As shown, a cryopreservation device using the electromagnetic freezing method includes a low-temperature constant temperature tank 1, a Helmholtz coil 2, a stabilized power supply 4, and a cooling platform 5; wherein, the low-temperature constant temperature tank 1 is connected to the cooling platform 5 through a circulation pump, The Helmholtz coil 2 is arranged on both sides of the cooling table 5, and the stabilized power supply 4 is connected to the Helmholtz coil 2. The cooling table 5 is made of aluminum alloy with a thermal conductivity of 228W / (m·K). The cooling table 5 A groove 8 is provided on the top, and the vial 3 containing medicine or organism is placed in the groove 8 of the cooling table 5 .

[0036] Such as figure 2 , image 3 As shown, the inside of the cooling platform 5 is provided with a bent cooling liquid flow channel 9, the cooling platform is provided with a cooling liquid inlet 6 and a cooling liquid outlet 7, and the cooli...

Embodiment 3

[0045] Such as figure 1 As shown, a cryopreservation device using the electromagnetic freezing method includes a low-temperature constant temperature tank 1, a Helmholtz coil 2, a stabilized power supply 4 and a cooling platform 5; wherein, the low-temperature constant temperature tank 1 is connected to the cooling platform 5 through a circulation pump, The Helmholtz coil 2 is arranged on both sides of the cooling table 5, the stabilized power supply 4 is connected to the Helmholtz coil 2, the cooling table 5 is made of aluminum alloy with a thermal conductivity of 228W / (m K), and the cooling table 5 A groove 8 is provided on the top, and the vial 3 containing medicine or organism is placed in the groove 8 of the cooling table 5 .

[0046] Such as figure 2 , image 3 As shown, the inside of the cooling platform 5 is provided with a bent cooling liquid flow channel 9, the cooling platform is provided with a cooling liquid inlet 6 and a cooling liquid outlet 7, and the cooling ...

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PUM

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Abstract

The invention discloses a low temperature preservation device with an electromagnetic freezing method applied, and an operation method of the low temperature preservation device. The low temperature preservation device comprises a low temperature and constant temperature tank, Helmholtz coils, a stabilized voltage supply and a cooling table, wherein the low temperature and constant temperature tank is connected with the cooling table through a circulating pump, the Helmholtz coils are distributed on the two sides of the cooling table, the stabilized voltage supply is connected with the Helmholtz coils, the cooling table is provided with a groove, and penicillin bottles filled with drugs or organisms are placed in the groove of the cooling table. The electromagnetic freezing method is utilized for storing the drugs or the organisms at a low temperature, and the integrity and timeliness of the stored drugs or the stored organisms are guaranteed.

Description

technical field [0001] The invention relates to a cryopreservation device using electromagnetic freezing method and an operation method thereof. Background technique [0002] Most modern medicines are heat-sensitive medicines, that is, medicines that are sensitive to temperature (mainly high temperature). In the production of heat-sensitive medicines, in order to prevent the denaturation of medicines due to high temperature and affect the quality of products, it is necessary to store medicines at low temperature. And many organisms also need to preserve their activity at low temperatures. How to avoid the damage in the process of freezing and rewarming to the greatest extent is the goal that people have been pursuing for a long time. At present, there are two main methods of cryopreservation technology, namely slow freezing method and vitrification method. The cooling rate of the slow freezing method is relatively slow, and a certain amount of cryoprotectant is added. So...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): F25D3/00A01N1/02
Inventor 赵红霞李文博韩吉田陈常念
Owner SHANDONG UNIV
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