Isaria fumosorosea
A technology of Corynebacterium coryneformis and Corynebacterium cortiformis, which is applied in the field of microorganisms and can solve the problems of unstable control effect and poor pest control effect.
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Embodiment 1
[0028] The acquisition of embodiment 1 fume-colored I. spp. strain SCAU-IFCF02
[0029] 1. Experimental materials and conditions
[0030] (1) A house termite infested by entomogenic fungi ( Coptotermes formosanus ) worm corpses.
[0031] (2) Potato dextrose agar medium (PDA): 200g potato, 17-20g agar, 20g glucose, 1000mL water, aliquoted, autoclaved (121°C, 30min).
[0032](3) Sterile operating conditions: All utensils and utensils must be sterilized in an autoclave (121°C, 30min), and inoculation and other operations are carried out in an ultra-clean workbench.
[0033] (4) Strain culture conditions: culture in a light incubator (14L: 10D) at 25±1°C, transfer to a PDA slope after colony formation, and then transfer to a 4°C refrigerator for storage.
[0034] 2. Isolation and identification of pathogenic bacteria
[0035] (1) Isolation: Isolate the pathogenic bacteria from the collected samples of house termite carcasses infected by entomogenic fungi. Use 5% sodium hyp...
Embodiment 2
[0061] Example 2 Determination of the pathogenicity of Isora fumoscens SCAU-IFCF02 strain to Plutella xylostella
[0062] Bioassay is one of the most important techniques in the theoretical and applied research of insect mycology and entomopathology, and it is one of the important means to detect the degree and rate of lethality of entomogenic fungi to target pests. It provides an important reference for the biocontrol potential of sporospora SCAU-IFCF02 strain. In the present invention, the pathogenicity of the strain I. fumigatus SCAU-IFCF02 to Plutella xylostella is measured to determine the optimal concentration and optimal age of the strain infecting Plutella xylostella, so as to guide the use of the bacterial strain in preventing and controlling field pests. in the application.
[0063] 1. Test materials
[0064] (1) Insects tested: diamondback moth Plutella xylostella (L.) Mature larvae were collected from the vegetable kale fields near South China Agricul...
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