Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing frankincense selenium-rich blueberry vinegar by means of yeast display of lipase

A technology of blueberry vinegar and lipase, which is applied in the field of food processing, can solve the problems that yeast has not successfully displayed rice lipase, cannot guarantee the successful display of lipase, has enzyme activity, and is expensive

Inactive Publication Date: 2014-04-30
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, on the one hand, due to poor stability and high price, lipase mainly relies on imports in my country, which limits the large-scale use of lipase; Rice lipase is difficult to separate from rice, and it has not been practically used, and it has been regarded as a harmful substance, which has been inactivated and discarded.
[0005] In recent years, the rapid development of modern biotechnology has brought the development and utilization of lipase to a new level, such as the doctoral thesis (Liu Wenshan. Research on the surface display of lipase in Saccharomyces cerevisiae [D] Wuhan, Huazhong University of Science and Technology, 2010 ), the surface display of lipase on yeast has the advantages of increasing the yield of lipase and not requiring separation and purification during use. Report on Rice Lipase and Its Application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing frankincense selenium-rich blueberry vinegar by means of yeast display of lipase
  • Method for producing frankincense selenium-rich blueberry vinegar by means of yeast display of lipase
  • Method for producing frankincense selenium-rich blueberry vinegar by means of yeast display of lipase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. Pichia pastoris displays rice lipase

[0015] 1. The Rlipase gene synthesis was entrusted to a biological company (in this case, Nanjing GenScript Biotechnology Co., Ltd.), and the gene was cloned on the pUC57 vector (pUC57-Rlipase). The DNA sequence is shown in SEQ1.

[0016] 2. Rlipase was subcloned into pPIC9K vector

[0017] The pUC57-Rlipase and pPIC9K vectors were digested with Eco RI and Not I respectively, and the enzyme digestion reaction systems were as follows:

[0018] pUC57-Rlipase plasmid 34 ml

[0019] 10* buffer 4ml

[0020] Eco RI 1 ml

[0021] Not I 1 ml

[0022] A total of 40ml system,

[0023] pPIC9K plasmid 6 ml

[0024] 10* buffer 2 ml

[0025] Eco RI 1 ml

[0026] Not I 1 ml

[0027] wxya 2 O 10ml

[0028] 20 ml system in total,

[0029] Mix them separately and place them in a water bath at 37°C for 4 h, then identify them by 1.2% agarose gel electrophoresis, the results are as follows: figure 1 shown.

[0030] 3. Recover the targ...

Embodiment 2

[0078] Example 2 The deployment of frankincense selenium-enriched blueberry vinegar

[0079] 1. Pichia pastoris displaying rice lipase: same as in Example 1.

[0080] Two, the making of selenium-enriched blueberry vinegar: with embodiment 1.

[0081] 3. The deployment of frankincense selenium-enriched blueberry vinegar

[0082] Such as image 3 Mix 20% of flavor-enhancing emulsion and 80% of selenium-enriched blueberry vinegar by weight → blending adjustment → add 0.1% sodium benzoate to the vinegar liquid → pack and sterilize → obtain the finished vinegar.

[0083]

Embodiment 3

[0084] Example 3 Preparation of frankincense selenium-enriched blueberry vinegar

[0085] 1. Pichia pastoris displaying rice lipase: same as in Example 1.

[0086] Two, the making of selenium-enriched blueberry vinegar: with embodiment 1.

[0087] 3. Blending of frankincense selenium-enriched blueberry vinegar

[0088] Such as image 3 As shown, mix 30% of flavor-enhancing emulsion and 70% of selenium-enriched blueberry vinegar by weight → blending adjustment → add 0.1% sodium benzoate to the vinegar liquid → pack and sterilize → obtain the finished vinegar.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for producing frankincense selenium-rich blueberry vinegar by means of yeast display of lipase. The method comprises the following steps: displaying rice lipase on the surface of pichia pastoris, performing multiplication culture for 48-96 hours to increase the yield of the rice lipase greatly along with the yield of the pichia pastoris, adding 1-4 parts by weight (wet weight) of the yeast on which the rice lipase is displayed into 200-300 parts of cream, carrying out an esterlysis reaction at 35-55 DEG C for 2-6 hours, and filtering to obtain aroma-enhanced emulsion; producing blueberry vinegar rich in organic selenium from selenium-rich blueberry and selenium-rich yeast serving as basic raw materials by adopting a semi-solid method, and mixing the aroma-enhanced emulsion and the selenium-rich blueberry vinegar in the ratio of 1:9-3:7 (w / w) to obtain the a frankincense selenium-rich blueberry vinegar health-care beverage which is deeply loved by customers. By using the frankincense selenium-rich blueberry vinegar, trace element necessary for human bodies, namely, selenium can be replenished safely and efficiently for customers, and the health-care functions of eyesight improving, skin moistening and ageing resisting of the blueberry vinegar are brought into play.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a method for utilizing lipase displayed on the surface of yeast to esterify fat for aroma enhancement and selenium-enriched yeast to produce frankincense selenium-enriched blueberry vinegar. Background technique [0002] Fruit vinegar has both the nutritional and health functions of fruit and vinegar, and is a drink that is popular with consumers and integrates functions such as nutrition, health care, and dietotherapy; and blueberry is known as the "Queen of Fruits". The blueberry vinegar disclosed by the Chinese patent of CN2475343A has the functions of eliminating eye fatigue, nourishing the skin, and delaying aging. [0003] Selenium is an essential trace element for the human body. 72% of my country's regions are selenium-deficient or low-selenium regions, which seriously threaten people's health. The development of blueberry vinegar drinks rich in frankincense and s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12J1/08C12J1/04
Inventor 谢定朱婧盛敏焦玲刘瑞兴李晓文谢易真
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com