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Hydrogel used as skin filling material and preparation method thereof

A filling material and hydrogel technology, applied in medical science, prosthesis, etc., can solve the problems of damaged tissue, chronic inflammation of tissue, and poor biocompatibility between gel and tissue, so as to enhance skin elasticity, biological High potency and good stability

Inactive Publication Date: 2014-04-30
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both of the above two types of products have the defects of chronic inflammation, tumor and tissue damage to the tissue, and the gel does not have good biocompatibility with the tissue, so it cannot fully meet the requirements of the body
The third-generation product, injectable hydrogel, has also been developed to a certain extent as an excellent filling material. Although it overcomes the shortcomings of tissue damage, it cannot fully meet the requirements of repair.

Method used

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  • Hydrogel used as skin filling material and preparation method thereof
  • Hydrogel used as skin filling material and preparation method thereof
  • Hydrogel used as skin filling material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Step 1: Weigh human-like collagen and dissolve it in double distilled water, then add chitosan solution to the human-like collagen solution, so that the mass ratio of human-like collagen to chitosan is 1:5, and the reaction temperature is controlled as -4°C, react under stirring conditions for 0.5h.

[0052] Among them, human-like collagen is human-like collagen produced by high-density fermentation of genetically engineered bacteria BL21; chitosan is chitosan with a deacetylation degree of more than 90%, and its mass volume fraction is 40 mg / mL.

[0053] Step 2: adding hyaluronic acid solution, the mass ratio of hyaluronic acid to chitosan is 0.1:1, the reaction temperature is controlled at 20°C, and the reaction is carried out for 0.2h under stirring condition.

[0054] Wherein, the hyaluronic acid is medical-grade hyaluronic acid with a molecular weight of 1,000,000 Da, and the mass fraction of the hyaluronic acid solution is 2%.

[0055] Step 3: add a cross-linking...

Embodiment 2

[0059] Step 1: Weigh human-like collagen and dissolve it in double distilled water, then add chitosan solution to the human-like collagen solution, so that the mass ratio of human-like collagen to chitosan is 3:5, and the reaction temperature is controlled as -2°C, react under stirring conditions for 0.5h.

[0060] Among them, human-like collagen is human-like collagen produced by high-density fermentation of genetically engineered bacteria BL21; chitosan is chitosan with a deacetylation degree of more than 90%, and its mass volume fraction is 40 mg / mL.

[0061] Step 2: adding hyaluronic acid solution, the mass ratio of hyaluronic acid to chitosan is 0.2:1, the reaction temperature is controlled at 25° C., and the reaction is carried out for 0.3 h under stirring conditions.

[0062] Wherein, the hyaluronic acid is medical-grade hyaluronic acid with a molecular weight of 1,000,000 Da, and the mass fraction of the hyaluronic acid solution is 2%.

[0063] Step 3: add a cross-lin...

Embodiment 3

[0067] Step 1: Weigh human-like collagen and dissolve it in double distilled water, then add chitosan solution to the human-like collagen solution, so that the mass ratio of human-like collagen to chitosan is 6:5, and the reaction temperature is controlled as 0°C, reacted for 1h under stirring conditions.

[0068] Among them, human-like collagen is human-like collagen produced by high-density fermentation of genetically engineered bacteria BL21; chitosan is chitosan with a deacetylation degree of more than 90%, and its mass volume fraction is 40 mg / mL.

[0069] Step 2: adding hyaluronic acid solution, the mass ratio of hyaluronic acid to chitosan is 0.4:1, the reaction temperature is controlled at 30° C., and the reaction is carried out under stirring condition for 0.5 h.

[0070] Wherein, the hyaluronic acid is medical-grade hyaluronic acid with a molecular weight of 1,000,000 Da, and the mass fraction of the hyaluronic acid solution is 2%.

[0071] Step 3: adding a cross-li...

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Abstract

The invention relates to a hydrogel used as a skin filling material and a preparation method thereof. Skin filling materials such as white wax, silicon resin, a saline water bag, and silica gel have defects of generating chronic inflammation, lump, and tissue damage to tissues, are poor in biocompatibility, and are incapable of meeting the recovering requirement. The preparation method comprises the steps: adding a chitosan solution in a human-like collagen protein solution, stirring for reacting, adding a hyaluronic acid solution, stirring for reacting, adding a crosslinking agent, and stirring for reacting; and heating by adopting a constant-temperature water bath to prepare the hydrogel, wherein a human-like collagen protein is produced by a genetically engineered bacterium BL21 high-density fermentation. The hydrogel provided by the invention is prepared by adopting two methods of self-assembling and physical / chemical crosslinking, and has the advantages of better stability and high biological value, is capable of preventing trauma of surgical operation and reducing patient pain, thereby actually realizing the advantage of skin filling.

Description

technical field [0001] The invention relates to a hydrogel, in particular to a hydrogel used as a skin filling material and a preparation method thereof. Background technique [0002] Hydrogel is a polymer network system, which is soft in nature, can maintain a certain shape, and can absorb a large amount of water. Human facial cosmetic plastic surgery mainly includes correcting wrinkles, improving facial contours, filling sunken scars or lips, and repairing injured tissues. It can retain moisture well in the environment, and has good viscosity, elasticity and softness. When the hydrogel is transplanted into the organism, it is difficult to meet the repair effect due to the different degrees of tissue damage; when the hydrogel is injected into the organism, if the hydrogel is environment-sensitive, such as temperature-sensitive, in the It is in the form of a sol at room temperature. When the temperature reaches the temperature of the human body, the hydrogel can form a ge...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08J3/24C08L89/00C08L5/08A61L27/24A61L27/20A61L27/52
Inventor 李贤范代娣马晓轩
Owner NORTHWEST UNIV
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