Method for evaluating immunization effect of vaccine used in breeder birds and egg birds based on egg laying protection index
An evaluation method and technology of immune effect, applied in the field of preventive veterinary medicine, can solve problems such as quantitative evaluation of discomfort, and achieve the effect of quick evaluation and accurate evaluation method
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Embodiment 1
[0014] Derivation of Calculation Formula of Egg Production Protection Index
[0015] The egg production protection index calculation formula of the present invention is deduced and obtained by the following method:
[0016] 1. Concept
[0017] 1.1 Protection index
[0018] The protection index usually refers to the ability of drugs or vaccines to protect the host against pathogenic microorganisms. It is usually used to evaluate the effect of drugs or vaccines prepared from pathogens that can cause animal disease (or death) to protect animals against pathogenic microorganisms. In terms of biological vaccines, its The calculation formula is:
[0019] Protection index (PI) = (mortality rate of the challenge group - fatality rate of the immune challenge group) / case fatality rate of the challenge group × 100%
[0020] 1.2 Egg production protection index
[0021] After the breeding (egg) poultry is infected by pathogens such as avian Tembusu virus or egg-laying syndrom...
Embodiment 2
[0047] Taking the poultry Tembusu virus inactivated vaccine as an example, the implementation of the vaccine immune effect evaluation system based on egg production protection index is described in detail:
[0048] The pathogen used for the challenge is poultry Tembusu virus, and the vaccine is an oil emulsion inactivated vaccine emulsified with an adjuvant after the virus is inactivated.
[0049] 90 160-day-old healthy shelduck ducks (both of which were negative for poultry Tembusu virus and its antibodies) were used as experimental animal models. The egg production rate of group B was similar), and the egg-laying ducklings of group B were immunized with the prepared poultry tambushu virus oil emulsion inactivated vaccine through the leg muscles, with a single dose of 1ml per duck, and groups A and C were vaccinated through the leg muscles. Intramuscularly inject 1 mL of oil emulsion prepared with physiological saline as the aqueous phase into the legs. On the 28th day after...
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