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Rapid in-vitro propagation method of saline alkali tolerant fast-growing Ulmus pumila

A salt-alkali-tolerant and white elm technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of low growth rate of culture medium, high cost, resources, waste, etc., to reduce production costs and grow The effect of vigorous, stable offspring traits

Inactive Publication Date: 2014-04-30
SHANDONG FOREST SCI RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The value-added rate of the above-mentioned medium is low, resulting in high cost and waste of resources in industrial production of saline-alkali-tolerant fast-growing Ulmus

Method used

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  • Rapid in-vitro propagation method of saline alkali tolerant fast-growing Ulmus pumila
  • Rapid in-vitro propagation method of saline alkali tolerant fast-growing Ulmus pumila
  • Rapid in-vitro propagation method of saline alkali tolerant fast-growing Ulmus pumila

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Experimental program
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Effect test

Embodiment 1

[0022] (1) Collection of explants of Ulmus clonal line: saline-alkali-tolerant and fast-growing Ulmus explants are collected in the morning on sunny days in spring (the season when explants just germinate); select young tissues or organs; wrap them in wet gauze , placed in a low-temperature preservation box, and brought back to the laboratory;

[0023] (2) Disinfection of explants: first, trim the explants of the Ulmus clonal line, retain the buds and growth points; scrub the surface with a soft brush without damaging the explants; use a low concentration of less than 1% Rinse with washing powder water 2-3 times (to remove the soil, floating and sinking, etc. on the surface of the explant), then rinse with running tap water for 30 minutes; transfer to a super-clean workbench, soak in 75% alcohol for 30 seconds, and rinse with sterile water 3-4 times, then soak in 0.1% mercury liter for 10 minutes, rinse with sterile water 4-5 times;

[0024] (3) Explant inoculation: cut off a...

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Abstract

The invention discloses a rapid in-vitro propagation method of saline alkali tolerant fast-growing Ulmus pumila. The rapid in-vitro propagation method comprises the steps of collecting clonal line explant of Ulmus pumila, disinfecting, inoculating, and subculturing, wherein inoculation and subculture are carried out in EM medium developed by the invention, the culture temperature is 25+ / -2DEG C at daytime (or under illumination condition) and 18+ / -2DEG C at night (or under dark condition), the illumination time is 12h per day, and the humidity is over 90%. By use of the culture medium and method of the invention, the average high proliferation rate of Ulmus pumila aseptic seedling is increased by more than 5 times, and the average proliferation coefficient is increased by more than 2.3 times.

Description

technical field [0001] The invention relates to a method for in vitro propagation of Ulmus alba, in particular to a method for in vitro rapid propagation of saline-alkali-tolerant fast-growing Ulmus alba. Background technique [0002] White elm (Ulmus pumila L.), a plant of the genus Ulmus in the family Ulmaceae, has many varieties, and elm has always been the top five native garden tree species in northern my country [Populus, Salix, elm (Ulmus), Chinese pagoda tree (Sophora), Ailanthus (Ailanthus)], and one of the world's four major street greening tree species, with a wide range of applications. [0003] White elm is a native tree species with a long history of cultivation in my country. It is mostly used for afforestation in saline-alkali land and arid and barren areas because of its characteristics of drought resistance, high temperature resistance, severe cold resistance, fast growth, good material, resistance to barrenness, and salt-alkali resistance. White elm is tou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 慕徳宇房用慕宗昭梁玉王月海王强
Owner SHANDONG FOREST SCI RES INST
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