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Preparation method and application of mirabilis jalapa linnaeus extract for controlling agricultural pest mites

A mirabilis extract, agricultural technology, applied in the directions of botanical equipment and methods, applications, acaricides, etc., can solve the problems such as the use of the mirabilis extract has not yet been found, achieve difficult drug resistance, expand the scope of application, and simple extraction easy effect

Inactive Publication Date: 2014-04-23
SHENYANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the use of mirabilis extract in the control of agricultural pests has not been found

Method used

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  • Preparation method and application of mirabilis jalapa linnaeus extract for controlling agricultural pest mites
  • Preparation method and application of mirabilis jalapa linnaeus extract for controlling agricultural pest mites
  • Preparation method and application of mirabilis jalapa linnaeus extract for controlling agricultural pest mites

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0021] The acaricidal activity of Mirabilis jasmine extract on Tetranychus urticae and Cinnamomum cinnabis is used to measure the contact biological activity of harmful mites by using the standard method of acaricide recommended by FAO --- glass slide immersion method.

[0022] Experimental Materials:

[0023] The Mirabilis mirabilis extract of 95% ethanol was obtained as follows: 500 grams of dried Mirabilis mirabilis whole herb was crushed, passed through a 40-mesh sieve, leached with 5 times the amount of 95% ethanol at room temperature for 4 days, and filtered to obtain an extract. Add 5 times the amount of 95% ethanol to the filter residue for leaching, and filter. After doing this three times, the three extracts were combined and concentrated under reduced pressure to obtain the Mirabilis mirabilis extract of the present invention with a yield of 10.8%. Then weigh 0.5g of the above-mentioned mirabilis extract, add 1% of the total volume of the extract in acetone to diss...

experiment example 2

[0034] The test of ovicidal activity of Mirabilis extract on Tetranychus urticae and Tetranychus cinnabarinus, the extracts and spider mites used in the experiment were prepared the same as in Experimental Example 1.

[0035] experimental method:

[0036] Refer to the standard method of ovicide recommended by FAO—leaf residual poison method to measure the ovicidal biological activity of Tetranychus urticae with slight modifications. Collect fresh cowpea leaves, cut off the petiole by 0.2 cm, wrap the petiole with wet cotton balls, put it in a petri dish with a layer of wet cotton, cover both sides of the leaves with wet cotton to make the leaves close to the cotton, and use zero Pick up 10 female adult mites to each leaf with a No. 1 soft brush, and let them lay eggs at 25±1°C. After 4 hours, remove the female adult mites, check and record the number of eggs, keep 30 mite eggs on each leaf, and place them in the In an intelligent artificial climate box at 25°C, measure when ...

experiment example 3

[0042] Experiment on the oviposition avoidance effect of mirabilis extract on female adult mites of Tetranychus urticae and Tetranychus cinnabarinus:

[0043]Collect fresh bean leaves, wash and dry them, soak 2 clean bean leaves in the test liquid and the control liquid respectively for 10 seconds, take out the leaves, let them dry naturally, flatten the leaves down and place them in parallel on a bed lined with a layer of wet cotton. In the petri dish, the petiole was wrapped with absorbent cotton, and tap water was used to keep it moist. The gap between the two leaves is about 0.5-1 cm. Connect two bean leaves with a cover glass, and surround the leaf and the cover glass with wet absorbent cotton strips to prevent the escape of Tetranychus urticae, while ensuring that the cover glass is dry and anhydrous. Take 15 adult female mites with a No. 0 soft brush and place them on the cover glass to ensure that they can crawl normally on the cover glass, and allow them to freely ch...

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Abstract

The invention discloses a preparation method and application of a mirabilis jalapa linnaeus extract for controlling agricultural pest mites, relating to a preparation method and application of an extract for controlling the agricultural pest mites. The preparation method comprises the following steps: drying whole mirabilis jalapa linnaeus in the shade, grinding the dried mirabilis jalapa linnaeus by a plant grinder, and filtering to obtain extract liquid; filtering residues, extracting with ethanol, and concentrating under reduced pressure by using a rotary evaporator to obtain black brown thick paste, i.e. the mirabilis jalapa linnaeus extract; or drying the whole mirabilis jalapa linnaeus in the shade, grinding the dried mirabilis jalapa linnaeus by the plant grinder, screening the ground mirabilis jalapa linnaeus through a 40-mesh sieve, adding 2-10-fold diluted 95% ethanol, performing reflux extraction for 5-15 hours, filtering to obtain reflux extract liquid, and concentrating under reduced pressure by using the rotary evaporator to obtain black brown thick paste, i.e. the mirabilis jalapa linnaeus extract. The mirabilis jalapa linnaeus extract is a botanical pesticide with a mite-killing activity and can also serve as a conventional auxiliary material required for a pesticide preparation to prepare a conventional pesticide preparation, including missible oil, microemulsion, suspension and an aqueous solution as an extract for controlling agricultural pest mites.

Description

technical field [0001] The invention relates to a preparation method and application of an extract of Mirabilis mirabilis for preventing and controlling agricultural pests, in particular to a preparation method and application of an extract for preventing and controlling agricultural pests. Background technique [0002] Herbivorous mites are the most common plant pests, which can seriously reduce the yield of many important economic crops, such as fruits, cotton, vegetables and ornamental plants, and are recognized as the most difficult pest community to control. Chemical pesticides have played an important role in controlling harmful mites in agricultural production, protecting crops from damage and ensuring agricultural economy. However, the long-term use of chemical pesticides has brought various disadvantages, such as excessive pesticide residues in agricultural products, and increased human and animal poisoning incidents. Pests have become resistant to pesticides, t...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01P7/02
Inventor 韩俊艳刘广纯邢艳艳王军聂争飞
Owner SHENYANG UNIV
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