Tissue culture rapid propagation method for anisetree barks

Abstract

The invention discloses a tissue culture rapid propagation method for anisetree barks, which comprises the following steps: (1) taking an anisetree bark seed as an explant, and disinfecting the anisetree bark seed; (2) placing the disinfected explant in an MS basic culture medium to carry out induced germination, so that a sterile test-tube plantlet is obtained; (3) placing the sterile test-tube plantlet in an MS propagation culture medium to carry out rapid propagation culture, so that a cluster bud is obtained; (4) placing the cluster bud in an MS strong seedling culture medium to carry out strong seedling culturing, so that a robust plant is obtained; (5) placing the robust plant in an MS rooting culture medium to culture, so that a complete root-carrying seedling is obtained; and (6) taking the complete root-carrying seedling to carry out hardening, transplanting the obtained seedling to a sand bed to grow for a month, and transplanting the seedling to a field. The growth coefficient of anisetree bark cluster buds obtained by using the culture method disclosed by the invention reaches 8-12 times, the rooting rate of the obtained tissue culture seedling is over 85%, and the survival rate of seedlings transplanted to a seedbed is over 90%, thereby effectively solving the large-scale seedling raising problem of anisetree barks.

Description

technical field [0001] The invention relates to a method for plant propagation, in particular to a rapid propagation method for tissue culture of maple bark. Background technique [0002] Illicium difengpi B.N Chang et al. is a plant of the genus Star Anise in Magnoliaceae. Its dry bark is used for medicinal purposes. It has the functions of expelling wind and dampness, promoting qi and relieving pain. The treatment of bruises and other symptoms has good curative effect and high medicinal value. It is the main raw material of various Chinese patent medicine products. After investigation, it was found that the maple bark mostly grows on the top of the mountain or under the sparse forest of the rocky mountain in the limestone area. The distribution area of ​​its habitat is very narrow, and the reserves of wild resources are scarce. As a result, land productivity in karst areas continued to decline, and the area of ​​rocky desertification continued to expand. The living enviro...

AI Technical Summary

Problems solved by technology

[0003] Under natural conditions, maple bark is mainly propagated by seeds, but because the seed coat is thin, the oil of the seeds will deteriorate when it is dry, and it will rot easily when it is too wet, and it is easy to lose germination power, and its growth environment is rocky. The thin soil cannot provide an ideal growth environment for the normal reproduction of maple bark seeds, resulting in very low reproductive capacity of maple bark in the natural environment, and the resource renewal rate is very slow, which is difficult to meet market needs

Although some scientific researchers have conducted some research on the cutting propagation technology of maple bark, the effect is not very obvious and cannot meet the needs of artificial cultivation.