Chemiluminescence detection kit for detecting clenbuterol hydrochloride

A clenbuterol hydrochloride and chemiluminescence detection technology, applied in the field of immunodetection, can solve the problems of lack of detection instruments, complicated instruments, long detection time, etc., and achieve good safety, high sensitivity, and long duration of light signal. Effect

Active Publication Date: 2015-12-30
LUOYANG LAIPSON INFORMATION TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantage is that the detection takes a long time and the instrument is complicated. At present, there is a lack of suitable and supporting detection instruments.

Method used

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  • Chemiluminescence detection kit for detecting clenbuterol hydrochloride
  • Chemiluminescence detection kit for detecting clenbuterol hydrochloride
  • Chemiluminescence detection kit for detecting clenbuterol hydrochloride

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specific Embodiment

[0041]The specific antigen of clenbuterol hydrochloride was coupled with bovine serum albumin (BSA) and used as the antigen for coating. The antigen solution was detected to have a clear appearance, no foreign matter visible to the naked eye, and no precipitate that would not disperse when shaken. The purity was high (average >99%), and has high titer and good activity, good specificity and sensitivity, it can be used to produce clenbuterol hydrochloride detection kit, according to the optimal working concentration of clenbuterol hydrochloride specificity Dilute the antigen with 0.05mol / L carbonic acid buffer solution at pH9.6, coat the chemiluminescent plate with 100μl per well, incubate at 2-8℃ for more than 20 hours, wash the plate once, add 150μl blocking solution to each well at 2-8 Incubate at ℃ for more than 20 hours, blot up the blocking solution and dry in an environment with a relative humidity of ≤30% for 5 hours, seal the package in an aluminum foil bag, and store a...

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Abstract

The invention relates to a chemiluminescent detection kit for detecting clenbuterol hydrochloride, which comprises a luminescent plate, a clenbuterol hydrochloride polyclonal antibody, an enzyme-labeled secondary antibody (horseradish peroxidase-labeled goat anti-rabbit antibody), gram hydrochloride Renbuterol series standard solution, concentrated phosphate buffer solution, concentrated washing solution, luminescent substrate A, luminescent substrate B, negative control and positive control; the clenbuterol hydrochloride chemiluminescence detection kit of the present invention has high sensitivity, Its sensitivity can reach 0.05ng / ml. Chemiluminescence immunoassay can detect substances that cannot be detected by radioimmunoassay and diagnostic analysis. It has a wide detection range, simple and fast operation, and is non-toxic and pollution-free. very important.

Description

technical field [0001] The invention relates to the field of immunoassay, in particular to a chemiluminescence detection kit for detecting clenbuterol hydrochloride. Background technique [0002] The quantitative detection techniques currently used to detect clenbuterol hydrochloride in urine, tissue, and serum of edible animals mainly include the following methods: [0003] 1. Gas chromatography-mass spectrometry (Gc-Ms) [0004] GC-MS can be used for qualitative and quantitative analysis of clenbuterol and other β-agonists in animal hair, urine and tissue. In the gas chromatography-mass spectrometry method for the determination of clenbuterol, the detection limit of CL in cow hair was 5ng / g. Positive biological materials were obtained by feeding rats with clenbuterol, and tested by O. Clenbuterol was extracted with 0.1 mol / L hydrochloric acid solution, degreased and purified with ether, extracted with ethyl acetate and evaporated to dryness, dissolved in ethanol and loa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N21/76
CPCG01N21/763G01N33/545
Inventor 王善普闫玉良刘姗姗刘欢王宇东
Owner LUOYANG LAIPSON INFORMATION TECH
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