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Reagent for consolidating soil by utilizing carbonic anhydrase microbes and using method thereof

A carbonic anhydrase and microbial technology, applied in the intersection of microorganisms and civil engineering materials, can solve the problems of air pollution, poor resources, slow growth of the strength of solidified soil, etc. Effect

Active Publication Date: 2014-04-16
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Traditional soil stabilizers are mainly cement and lime, which are increasingly facing the problems of poor resources, energy consumption, CO 2 Sustainable development issues such as emissions and air pollution, and its solidified soil strength increases slowly

Method used

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  • Reagent for consolidating soil by utilizing carbonic anhydrase microbes and using method thereof
  • Reagent for consolidating soil by utilizing carbonic anhydrase microbes and using method thereof
  • Reagent for consolidating soil by utilizing carbonic anhydrase microbes and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Weigh 12g of sucrose, Na 2 HPO 4 12H 2 O3g, MgSO 4 0.6g, CaCO 3 1.5g, KCl0.2g, (NH 4 ) 2 SO 4 Dissolve 0.6g and 0.4g of yeast extract in 1000mL deionized water, prepare the required medium solution, adjust the pH to 7, sterilize at 125°C for 25 minutes, take it out and wait for cooling, inoculate Bacillus colloidus to cooling culture In the base solution, shake culture at 30°C, the shaking frequency is 170r / min, and the culture time is 24h;

[0026] (2) Centrifuge the cultured bacterial liquid at high speed for 5 minutes, the centrifuge speed is 8000rpm, and the temperature is 4°C. Remove the nutrients in the upper medium, add 20mL of deionized water to make a concentrated bacterial liquid, and the bacterial cell concentration is 1×10 10 a / mL;

[0027] (3) Mix 1000g of dry soil, 20mL of highly concentrated colloidal bacillus bacteria solution, 200g of MgO, and 200g of water, stir well and fill it into a Φ10cm×15cm cylindrical test mold for compaction;

[00...

Embodiment 2

[0031] (1) Mix 1000g of dry soil, 200g of MgO, and 200g of water, stir well and fill it into a Φ10cm×15cm cylinder test mold for compaction;

[0032] (2) Inject CO with a volume concentration of 98% from the top of the test mold 2 Gas, the feeding rate is 5L / min, keep ventilation for 200min, and remove the mold.

[0033] The soil sample was solidified after 200 minutes of ventilation, and the compressive strength was 1.8MPa ( Figure 4 ), significantly lower than Example 1,; observed under the scanning electron microscope, the soil sample structure is relatively loose, and the cementation between particles is not obvious ( image 3 ).

Embodiment 3

[0035] (1) Weigh 9.6g of sucrose, Na 2 HPO 4 12H 2 O2.4g, MgSO 4 0.48g, CaCO 3 1.2g, KCl0.16g, (NH 4 ) 2 SO 4Dissolve 0.48g and 0.32g of yeast extract in 1000mL of deionized water, prepare the required medium solution, adjust the pH to 7, sterilize at 125°C for 25 minutes, take it out and wait for cooling, inoculate Bacillus colloidus to cooling culture In the base solution, shake culture at 30°C, the shaking frequency is 170r / min, and the culture time is 24h;

[0036] (2) Centrifuge the cultured bacterial liquid at high speed for 10 minutes, the speed of the centrifuge is 5000rpm, and the temperature is 4°C. Remove the nutrients in the upper medium, add 100mL of deionized water to make a concentrated bacterial liquid, and the concentration of the bacterial cells is 2×10 9 a / mL;

[0037] (3) Mix 1000g of dry soil, 100mL of high-concentration colloidal bacillus liquid, 100g of MgO, and 50g of water, stir well and fill it into a Φ10cm×15cm cylindrical test mold for compa...

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Abstract

The invention discloses a reagent for consolidating soil by utilizing carbonic anhydrase microbes and a using method thereof. The reagent comprises the following components in parts by mass: 1000 parts of dry soil, 50-200 parts of MgO and 50-200 parts of water. Highly concentrated bacillus mucilaginosus liquid is added according to the amount of 20-100mL / g, wherein the concentration of the highly concentrated bacillus mucilaginosus liquid is 109-1011per mL. The using method comprises the following steps: uniformly mixing and stirring the reagent, filling the reagent in a die trial for compacting, introducing CO2 gas from the top of the die trial, and keeping ventilating for 20-200 minutes, so that the cured and cemented soil sample can be obtained after die removal, and the compressive strength can be over 5MPa. A catalytic action of the carbonic anhydrase is mainly utilized, a hydration reaction of CO2 is promoted, a carbonate compound with cementing power is formed through mineralization, the cement solidification soil is replaced, and the reagent is energy-saving and environment-friendly.

Description

technical field [0001] The present invention relates to a kind of method utilizing carbonic anhydrase microbial solidification soil, mainly utilizes the catalysis of carbonic anhydrase, promotes CO 2 The hydration reaction of mineralization forms carbonate compounds with cementing ability, which replaces cement to solidify soil, and belongs to the cross-technical field of microorganisms and civil engineering materials. Background technique [0002] Traditional soil stabilizers are mainly cement and lime, which are increasingly facing the problems of poor resources, energy consumption, CO 2 Sustainable development issues such as emissions and air pollution, and its solidified soil strength increases slowly. Calcite formed in some specific environments in nature not only has high strength, but also has a certain degree of cementation. Inspired by this natural phenomenon, the present invention attempts to absorb CO through the enzyme acceleration and regulation of carbonic an...

Claims

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Application Information

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IPC IPC(8): C09K17/00C09K103/00
Inventor 王瑞兴钱春香
Owner SOUTHEAST UNIV
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