Method for building stenosis model after rat carotid artery balloon injuries

Abstract

The invention discloses a method for building a stenosis model after rat carotid artery balloon injuries. The method includes the steps of injecting pentobarbital sodium to the abdominal cavity of a rat to anesthetize the rat, removing furs to prepare the skin, enabling the rat to be fixed on an operation table in a supination mode, cutting the rat along the right middle line of the neck, exposing the left common carotid artery, disinfecting the skin at the position of the groin on the right side of the rat, separating the femoral artery, conducting ligation on the far-heart end at the position 1cm below the branch of the femoral artery, using a needle of a 1m1 injection syringe to puncture the femoral artery, feeding a super-smooth guide wire through the needle of the injection syringe into the carotid artery on the left side, feeding a balloon into the carotid artery on the left side along the super-smooth guide wire, feeding a balloon catheter into the bifurcated position of the internal carotid and external carotid artery of the left side of the neck, enabling the pressure in the balloon to be 1.0 barometric pressure to enable the balloon to right be capable of being pulled in the blood vessel, expanding the balloon to drag the balloon back and forth three times to strip the inner skin to enable the carotid artery to be damaged, withdrawing the balloon catheter, conducting ligation on the near-heart end of a notch of the artery, sewing the skin, and injecting penicillin into the muscle for continuous three days after an operation to avoid infections. According to the method, the operative difficulty in the prior art is reduced, the modeling success rate is improved, and the complication is reduced.

Description

technical field [0001] The invention belongs to the technical field of clinical medical research, and in particular relates to a method for establishing a rat carotid artery stenosis model after balloon injury. Background technique [0002] Atherosclerosis (AS) is the main pathological basis of human cardiovascular and cerebrovascular diseases. In recent years, the incidence of coronary atherosclerotic heart disease caused by atherosclerosis has increased significantly. Percutaneous coronary intervention (PCI) has become an important method for the treatment of coronary atherosclerotic heart disease. However, some patients with successful PCI treatment have coronary artery restenosis (restenosis, RS) is a major challenge faced by PCI treatment. There are two published technical solutions for the research and prevention of RS; [0003] Technical scheme one is " intraperitoneal injection of pentobarbital sodium (40mg / kg) anesthetized rats in rats, and 10mg / kg is added when ne...

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Problems solved by technology

[0014] The purpose of the embodiments of the present invention is to provide a method for establishing a model of rat carotid artery stenosis after balloon injury, aiming to solve the disadvantages of operating from the femoral artery and puncturing from the external carotid artery in the existing technology. Multiple injuries to the femoral artery lead to massive bleeding, which is inconvenient for balloon fixation and positioning

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