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Construction for Chromosome 1 to replace wild mus musculus strain C57BL/6. Zaozhuang 2-Chr1

A chromosome replacement, 2-chr1 technology, applied in the field of animal husbandry, etc., can solve the problem of unclear genetic characteristics

Inactive Publication Date: 2014-04-16
上海西普尔-必凯实验动物有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are few studies on Zaozhuang 2 mice, and its genetic characteristics are relatively unclear

Method used

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  • Construction for Chromosome 1 to replace wild mus musculus strain C57BL/6. Zaozhuang 2-Chr1
  • Construction for Chromosome 1 to replace wild mus musculus strain C57BL/6. Zaozhuang 2-Chr1
  • Construction for Chromosome 1 to replace wild mus musculus strain C57BL/6. Zaozhuang 2-Chr1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1, the construction method of chromosome replacement mouse

[0049]The embryos of Zaozhuang 2 mice were obtained from Shanghai Slack Experimental Animal Co., Ltd., and the cryovial containing the frozen embryos was taken out of the liquid nitrogen; the lid was opened, and after 30 seconds at room temperature, preheated 0.25M sucrose solution was added, and carefully pipetted for 10 First, move the mixture to a petri dish, then suck out the embryos with an egg washing needle, put them into the preheated M2 solution, let them rest for a while, wash them with M2 solution 5 times, transfer them into M16 solution, and cultivate them in the incubator for use. The embryos are transplanted into the uterus of the mother mouse and develop into mice of the same genotype as the frozen embryos.

[0050] Adult mice of Zaozhuang 2 (a wild Mus musculus obtained in Zaozhuang, Shandong) and C57BL / 6 were taken, Zaozhuang 2 was used as the male parent, and C57BL / 6 was used as th...

Embodiment 2、1

[0052] Example 2, No. 1 chromosome genome identification

[0053] 1. Primary Screening and Identification of Chromosome 1 Genome

[0054] Compare the gene sequences of chromosome 1 of the two strains of Zaozhuang 2 and C57BL / 6 (refer to the public information of MGI (http: / / www.informatics.jax.org / ) for the sequence), and select representative, two Different SNP sites of different strains, these SNP sites are evenly distributed on chromosome 1, and are easy to design and operate. Then design primers for these SNP sites to amplify sequences with characteristic SNP sites, and design the range of PCR amplified fragments between 100 and 400 bp. .mit.edu / primer3 / ) and the Oligo6.0 program (Molecular Biology Insights Inc., USA) were completed. Site-specific probes were designed for these SNP sites, and the length of the LDR probe ligation product was 80-130 bp.

[0055] Chromosomal SNP loci for Zaozhuang 2 and C57BL / 6 mice are shown in Table 1.

[0056] Table 1, selected SNP sit...

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PUM

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Abstract

The invention relates to construction for Chromosome 1 to replace wild mus musculus strain C57BL / 6. Zaozhuang 2-Chr1. A novel Chromosome C57BL / 6 replacing experiment musculus strain is constructed, a C57BL / 6 musculus is mainly used as the genetic background of the musculus strain, and a Zaozhuang 2 musculus is mainly used in the Chromosome 1. The Chromosome replacing strain can be used for studying character difference brought by different Chromosome 1 genomes under the background of the same genome, and a useful tool for studying the functions of the genomes by using a genetics method is achieved.

Description

technical field [0001] The invention belongs to the field of mouse strain construction, and in particular relates to the construction of No. 1 chromosome replacement wild Mus musculus C57BL / 6.Zaozhuang 2-Chr1. Background technique [0002] The lack of genetic diversity in experimental Mus musculus strains will cause great obstacles to the study of complex traits. This is mainly reflected in two aspects: first, the number of alleles at each locus in the population is small, and the number of alleles for polygenic related traits is relatively small. During the research, due to the similarity of the genetic background, the information of some trait-related genes will be missed; secondly, the linkage disequilibrium of the genome is relatively large, which is beneficial to the initial positioning of the trait-related genes, but it causes great difficulties for fine positioning. Resulting in consuming a lot of time and individuals. [0003] The living environment of wild Mus musc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
Inventor 赵莹肖君华邢正弘陈国强周宇荀李凯赵丽亚晁天柱柏熊高捷
Owner 上海西普尔-必凯实验动物有限公司
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