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A method for the simultaneous detection of multiple mycotoxins

A mycotoxin and isotope technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as simultaneous separation and matrix interference, and achieve the effect of breaking international trade barriers, short time consumption, and promoting import and export trade

Active Publication Date: 2015-09-16
SHANGHAI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] However, due to the similar chemical structures, chromatographic behaviors, and optical properties of different types of mycotoxins, common separation methods cannot separate them simultaneously in a short period of time, and the matrix interference caused by the complex components of agricultural products makes multiple mycotoxins simultaneously efficient. Detection is extremely challenging
At present, there is still a lack of analytical methods that can simultaneously detect multiple residues of six major types of mycotoxins in different edible agricultural products (corn, wheat, shiitake mushrooms)

Method used

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  • A method for the simultaneous detection of multiple mycotoxins
  • A method for the simultaneous detection of multiple mycotoxins
  • A method for the simultaneous detection of multiple mycotoxins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The samples of corn, wheat, and shiitake mushrooms collected in the market were analyzed and tested according to the following operation procedures.

[0045] Take samples of corn, wheat, and shiitake mushrooms, crush them, sieve them, and store them in a refrigerator at -20°C until use.

[0046] After the sample was taken out and returned to room temperature, 2 g of sample powder was placed in a 50 mL centrifuge tube, and 200 μL of the above five isotope internal standard mixture (50 ng mL -1 of 13 C-AFB1, 13 C-OTA, 13 C-T2 and 500ng mL -1 of 13 C-ZEN, 13 C-DON).

[0047] Let the sample added with isotope internal standard solution stand at room temperature for 1 hour, add 5 mL of water to soak for 5 minutes, sonicate for 40 minutes, then add 5 mL of acetonitrile solution containing 1% formic acid, vortex mix for 30 seconds, and sonicate for 40 minutes.

[0048] Add 2g of anhydrous magnesium sulfate and 0.5g of sodium chloride, shake vigorously for 30s immediatel...

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Abstract

The invention provides a method for simultaneously detecting various fungaltoxins. The method comprises the steps of extracting and purifying a sample; detecting purified liquid by a liquid chromatography tandem mass spectrometry technology; finally accurately measuring the fungaltoxins in an agricultural product by an isotope internal standard dilution method. The method for simultaneously detecting various fungaltoxins, which is disclosed by the invention, can be applied to simultaneous detection of 33 fungaltoxins in plants such as corns, wheat and lentinula edodes. The method disclosed by the invention has the characteristics of short time consumption, sensitivity and accuracy and can be applicable to simultaneous detection of a large batch of samples.

Description

technical field [0001] The invention relates to the technical field of agricultural product safety detection, in particular to a method for simultaneously detecting multiple mycotoxins. Background technique [0002] Due to the pressure of population and environment, the whole world is facing the double challenge of huge edible agricultural product supply and safety. According to the United Nations Food and Agriculture Organization (FAO), about 25% of the world's crops are polluted by mycotoxins every year, and about 2% of the crops lose their nutritional and economic value due to serious pollution, causing direct and indirect losses of tens of billions of dollars . Cereals and other crops, from the field to the dining table, have the risk of fungal contamination in every link of the industrial chain. These fungi may produce a series of toxic secondary metabolites with different chemical structures, called mycotoxins. Mycotoxin pollution has become a worldwide public safet...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 韩铮武爱波冯智红王建华
Owner SHANGHAI ACAD OF AGRI SCI
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