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Preparation method of fish collagen double sustained-release film

A double sustained-release, fish collagen technology is applied in the directions of pharmaceutical formulations, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc., to achieve the effects of simple and convenient preparation process, reduced toxic and side effects, and mild conditions.

Inactive Publication Date: 2014-04-09
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are few reports on the use of fish collagen as an implantable sustained-release drug carrier material.

Method used

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  • Preparation method of fish collagen double sustained-release film

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: A preparation method of fish collagen-based double sustained-release drug film

[0022] 1) Weigh 130 mg of GC and dissolve in 6 mL of distilled water, and dissolve 72.8 mg of CHS in 20 mL of 65% ethanol solution, then add 38.0 mg of EDC and 22.9 mg of NHS, stir for 10 h, then slowly add to the GC solution, at 40°C After stirring and reacting for 24 h, dialyze with 80% ethanol solution and distilled water for 24 h respectively, and freeze-dry to obtain CHGC; weigh 0.25 g of CHGC, 0.37 g of biotin, 0.20 g of EDC, and 0.12 g of NHS and dissolve them in 30 mL of DMSO The solution was reacted at 20°C for 24 h, dialyzed in distilled water for 48 h, and freeze-dried to obtain bio-CHGC.

[0023] 2) Dissolve 0.12 g bio-CHGC in 20% acetic acid solution, sonicate the probe for 2 min under ice bath conditions, ultrasonic power is 80 W, sonicate 5 times, and dialyze in distilled water for 24 h to obtain bio-CHGC self-aggregated nanoparticles grain. 800 μL of 5 mg / mL dox...

Embodiment 2

[0026] Example 2: A preparation method of fish collagen-based double sustained-release drug film

[0027] 1) Weigh 130 mg of GC and dissolve in 8 mL of distilled water, and dissolve 72.8 mg of CHS in 20 mL of 75% ethanol solution, then add 38.0 mg of EDC and 22.9 mg of NHS, stir for 10 h, then slowly add to the GC solution, at 40°C After stirring and reacting for 72 h, dialyze with 80% ethanol solution and distilled water for 24 h respectively, and freeze-dry to obtain CHGC; weigh 0.25 g of CHGC, 0.37 g of biotin, 0.20 g of EDC, and 0.12 g of NHS and dissolve them in 40 mL of DMSO The solution was reacted at 40°C for 24 h, dialyzed in distilled water for 48 h, and freeze-dried to obtain bio-CHGC.

[0028] 2) Dissolve 0.12g bio-CHGC in 20% acetic acid solution, sonicate the probe for 2 min under ice bath conditions, ultrasonic power is 80 W, sonicate 5 times, and dialyze in distilled water for 24 h to obtain bio-CHGC self-aggregated nanoparticles grain. Slowly add 1.6 mL of 5...

Embodiment 3

[0031] Example 3: In vitro release experiment of fish collagen-based double sustained-release drug film

[0032] Weigh a certain amount of fish collagen-based double slow-release drug film or drug-loaded nanoparticles into the dialysis bag, then completely submerge the sealed dialysis bag in 10 mL of PBS solution (pH 7.4), at 37°C, 110 rpm Shake in the dark under the conditions, take out all the release medium on 1, 3, and 5 days, and pour into an equal volume of fresh medium, measure the release amount of the drug at 480 nm with a UV-visible spectrophotometer and calculate the cumulative Release rate (Q%). Its formula is as follows:

[0033]

[0034] C n : T n The concentration of the sample at the time point, μg / mL; V: the total volume of the release medium, mL; V i : T i The volume of medium released at the time point, mL; C i : T i Concentration of samples at time points, μg / mL; W DBCN : mass of drug-loaded nanoparticles, μg; LC%: drug-loaded amount of drug-load...

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Abstract

The invention relates to a fish collagen double sustained-release film and a preparation method thereof. An anti-tumor drug is encapsulated by biotin-coupled cholesteric glycol chitosan self-aggregated nanoparticles, and then drug-loaded nanoparticles are compounded with waste fish skin collagen and chitosan, so as to prepare the fish collagen double sustained-release film in manners of freezing and drying. The film has the advantages that local slow release is carried out, toxic and side effects of the whole body are reduced, and the like.

Description

technical field [0001] The invention relates to a method for preparing a fish collagen-based double slow-release drug film, which belongs to the field of pharmaceutical preparations. Background technique [0002] Chemotherapy is one of the main means of treating malignant tumors, but the selectivity of anticancer drugs is low, and its severe side effects often cause great harm to patients. Therefore, there is an urgent need to find a drug dosage form that can maintain an effective drug concentration in the body for a long time, especially in the local tumor, so as to achieve high-efficiency and low-toxicity tumor treatment. The implantable sustained-release drug film is a new drug dosage form that meets this requirement. Combining the slow-release drug film with nanotechnology can improve the phenomenon of sudden release of drugs, realize double slow-release and targeting of drugs, thereby enhancing the anti-tumor effect of drugs and reducing toxic and side effects. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K47/36A61K9/51A61K31/704A61K31/203A61K31/337A61K31/4745A61K31/519A61P35/00
Inventor 张其清陈名懋郭豪黄玉清王建华方哲翔
Owner FUZHOU UNIV
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