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Application of pseudomonas aeruginosa strain

A technology of Pseudomonas aeruginosa and strains, applied in the fields of application, fungicides, chemicals for biological control, etc., can solve the problems of less research on plant pathogenic bacteria, and achieve the expansion of antibacterial spectrum, convenient operation method, and fermentation The effect of simple process

Active Publication Date: 2014-04-09
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the bacteria can produce a variety of antibacterial substances, the antibacterial substances produced by different strains are different, and the same strain can also produce a variety of antibacterial substances, and these antibacterial substances have inhibitory effects on plant pathogenic fungi, but their extracts and There are few studies on the inhibitory effect of Jinggangmycin compound on plant pathogenic fungi, and less research on plant pathogenic bacteria. Only its metabolite pyocyanin has inhibitory effect on bacteria, and other metabolites have research on plant pathogenic bacteria There is no report

Method used

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  • Application of pseudomonas aeruginosa strain
  • Application of pseudomonas aeruginosa strain
  • Application of pseudomonas aeruginosa strain

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Example 1 Preparation of strains of the present invention

[0031] The antagonistic bacteria were screened from the rice-duck co-culture field in Liuyang City by dilution separation method. Accurately weigh 10g of duck manure into 90mL of sterile water with glass beads, shake for 30min, stand for 10min, take the suspension, and dilute it to 1×10 according to the gradient concentration method. -7 times, and then spread the diluted solution on beef extract peptone agar medium plate and cultivate to obtain a single colony. The obtained single colony is made into a suspension with sterile water and made into a filter paper sheet, and is stored on the potato agar medium plate at the symmetrical 2cm place on both sides of the central rice sheath blight bacterium cake (5mm) and cultivated, and the indicator bacteria colony is cultivated. After the plate is covered, the bacterial colonies with the inhibition zone are picked and purified repeatedly to obtain a single colony, wh...

example 2

[0032] Example 2 Activation and fermentation of strains of the present invention

[0033] The strain SU8 of the present invention was inserted into the beef extract peptone slant medium (beef extract 3-6g, peptone 8-10g, sodium chloride 4-5g, glucose 15-20g, water 1000mL, pH 7.2-7.3) using an inoculation loop, Incubate at 28°C for 24h. Then, 10% of the inoculum was added to the beef extract peptone liquid medium, the rotational speed of the magnetic stirrer was 1000 r / min, and it was continuously cultured in a shaking incubator at 28-30 °C for 84 hours to obtain a fermentation broth.

example 3

[0034] Example 3 Extraction of antibacterial substances

[0035] The fermentation broth and ethyl acetate were fully mixed at a ratio of 1:2 for immersion and extraction for 48 hours, and the extract was placed in a rotary evaporator at a temperature of 40 °C and a rotating speed of 110 r / min to a paste to obtain antibacterial substances.

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Abstract

The invention belongs to the technical field of biopesticides and in particular relates to antagonistic bacteria SU8 (Pseudomonas aeruginosa). The Pseudomonas aeruginosa has been preserved at China center for type culture collection in May 7th, 2013, and the preservation number is CCTCC NO: M2013178. An ethyl acetate extract in the SU8 fermentation broth is compounded with validamycin to achieve the synergetic effect on preventing rice sheath blight disease; the ethyl acetate extract has an inhibiting effect on Xanthomonas oryzae pv. oryzicola and X. axonopodis pv. Citri.

Description

[0001] This application is a divisional application with an application number of 201310250195.0, an application date of June 21, 2013, and the title of the invention is a strain of Pseudomonas aeruginosa. technical field [0002] The invention belongs to the technical field of biological pesticides, particularly relates to antagonistic bacteria-Pseudomonas aeruginosa (Pseudomonas aeruginosa) SU8, and also relates to the application of the strain. Background technique [0003] In agricultural production, chemical pesticides are often used to prevent and control crop diseases. Although chemical pesticides have ideal control effects on crop diseases, the negative problems brought about by chemical pesticides have become increasingly prominent, such as polluting the environment, destroying natural enemies, and long-term use can easily induce pathogenic bacteria to develop drug resistance. Secondly, the excessive application of chemical pesticides not only increases the cost of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P1/00A01P3/00
Inventor 廖晓兰张亚苏品黄璜刘双清
Owner HUNAN AGRICULTURAL UNIV
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