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Culturing method capable of effectively reducing reverse mutation rate of horseradish polyploid plants

A technology of reverse mutation and cultivation method, which can be applied in the cultivation field that can effectively reduce the reverse mutation rate of wasabi polyploid plants, can solve the problems of plant polyploid induction failure, fast division speed, etc. rate effect

Active Publication Date: 2014-04-09
广元市玺府生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is mainly due to the fact that the undoubled diploid cells divide faster than the induced polyploid cells, so the diploid cells may eventually "flood" the polyploid cells during the growth of chimeric plants, As a result, the induced polyploid plants revert to diploid, which eventually leads to the failure of plant polyploid induction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Select the full-bodied wasabi seeds of the current year, first sterilize them with 0.01% peracetic acid for 10 minutes, then wash them twice with sterile water, and then store them at a low temperature of 3°C for 10 days;

[0021] (2) Place the wasabi seeds preserved at low temperature on filter paper soaked in a mixed solution of 5-aminouracil and propylene glycol and cultivate them for 10 hours at a temperature of 5°C. The mixed solution of 5-aminouracil and propylene glycol has a concentration of 0.5mmol.L -1 5-aminouracil solution and a concentration of 0.3mol.L -1 The propylene glycol solution is prepared in a volume ratio of 1:0.95;

[0022] (3) After the cultured wasabi seeds were washed twice with sterile water, they were placed at 12°C for recovery culture with sterile water for 5 hours, and then transferred to a place soaked in amisulfame solution and pentynchlor Cultivate on the filter paper of solution 3h, culture temperature is 4 ℃, and light intensit...

Embodiment 2

[0027] (1) Select the full-grown wasabi seeds of the current year, first sterilize them with 0.04% peracetic acid for 20 minutes, then wash them with sterile water for 3 times, and then store them at a low temperature of 6°C for 12 days;

[0028] (2) Put the wasabi seeds preserved at low temperature on the filter paper soaked in the mixed solution of 5-aminouracil and propylene glycol and cultivate them for 18 hours at a temperature of 8°C. 1.5mmol.L -1 5-aminouracil solution and a concentration of 0.5mol.L -1 The propylene glycol solution is prepared in a volume ratio of 1:1;

[0029] (3) After the cultured wasabi seeds were washed with sterile water for 3 times, they were placed at 15°C for recovery culture with sterile water for 7 hours, and then transferred to a place soaked in a mixed solution of amisulphazin and pentynchloride. Cultivate on filter paper for 7 hours, the cultivation temperature is 6°C, the light intensity is 2500lx, and the mixed solution of amisulphazi...

Embodiment 3

[0034] (1) Select the full-bodied wasabi seeds of the current year, first sterilize them with 0.07% peracetic acid for 30 minutes, then wash them with sterile water for 5 times, and then store them at a low temperature of 10°C for 15 days;

[0035] (2) Place the wasabi seeds preserved at low temperature on filter paper soaked in a mixed solution of 5-aminouracil and propylene glycol and cultivate them for 24 hours at a temperature of 9°C. The mixed solution of 5-aminouracil and propylene glycol has a concentration of 2.5mmol.L -1 5-aminouracil solution and a concentration of 0.7mol.L -1 The propylene glycol solution is prepared in a volume ratio of 1:1.05;

[0036] (3) After the cultured wasabi seeds were washed 5 times with sterile water, they were placed at 18°C ​​for recovery culture with sterile water for 9 hours, and then transferred to a place soaked in a mixed solution of amisulfame-lazin and pentynchloride. Cultivate on filter paper for 10h, the cultivation temperatu...

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PUM

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Abstract

The invention discloses a culturing method capable of effectively reducing the reverse mutation rate of horseradish polyploid plants. The method comprises the following steps: disinfecting horseradish seeds and sequentially culturing by a mixed solution of 5-aminuracil and propylene glycol, a mixed solution of oryzalin and propyzamide, and a mixed solution of colchicine and fumiron. With the culturing method, the reverse mutation rate of polyploid seedlings cultured by obtained polyploid seeds can be effectively reduced; the culturing method is a new way for rapidly culturing good horseradish varieties.

Description

technical field [0001] The invention relates to a method for tissue culture of wasabi plants, in particular to a culture method capable of effectively reducing the reversion rate of wasabi polyploid plants. Background technique [0002] Wasabi japonica Matsum (Wasabi japonica Matsum) is a perennial herbaceous semi-shady plant of the Brassicaceae family. [0003] The propagation of wasabi plants mostly adopts branch propagation and seed propagation. Ramet propagation not only has the problem of serious species degeneration after multi-generation vegetative propagation, but also has problems such as low reproduction coefficient and serious infection of seedlings, which can easily lead to large-scale occurrence of diseases. At present, wasabi resources are very scarce, far from meeting market demand, so it is necessary to cultivate high-quality new varieties of wasabi. Polyploid plants have the characteristics of organ gigantism, and due to the doubling of cell chromosomes, t...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王跃华孙雁霞段茂华任三军陈传凤彭双严幸林梅英徐恩琴唐凤如宋超刘银花唐川
Owner 广元市玺府生物科技有限公司
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