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Bacillus coagulans and preparation method and application in biological deodorant thereof

A technology of Bacillus coagulans and microorganisms, applied in biochemical equipment and methods, deodorization, microorganisms, etc., can solve the problems that have not been reported about Bacillus coagulans, achieve good antibacterial effect, facilitate production control, and eliminate invading bacteria Effect

Active Publication Date: 2014-04-02
SHENZHEN XINBAO ENVIRONMENTAL PROTECTION ENERGY SCI & TECH CO LTD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, there has been no report on the biological deodorization of Bacillus coagulans so far

Method used

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  • Bacillus coagulans and preparation method and application in biological deodorant thereof
  • Bacillus coagulans and preparation method and application in biological deodorant thereof
  • Bacillus coagulans and preparation method and application in biological deodorant thereof

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Embodiment

[0039] 1. Preparation method of biological deodorant

[0040] Under aseptic operation, pick a ring of Bacillus coagulans from the preservation slope, and inoculate it into the seed culture medium (water 100mL, sugar 1.0-2.5g, yeast extract 0.1-0.3g, bran filtered water 0.1, pH 5.5), cultured at 37°C for 48 hours at 200r / min to obtain seed solution.

[0041] Put the seed liquid into 10L sterile liquid fermentation medium according to the inoculation amount of 1%-3% (water 10mL, sugar 100-250g, yeast extract 10-30g, bran filtered water 100mL, pH 5.5, 121℃ Sterilize for 30min) using static culture method. Sampling is taken every 6 hours during the fermentation process, and the number of spores in the fermentation broth is detected by the method of gradient dilution and plate coating. When the number of spores reaches 1×10 8 When the fermentation is stopped at cfu / mL, the Bacillus coagulans liquid biological deodorant is obtained.

[0042] 2. Indoor simulated garbage deodorization effe...

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Abstract

The invention belongs to the environmental protection field, and discloses bacillus coagulans, a preparation method and an application in biological deodorants thereof. The bacillus coagulans of the invention were preserved in China General Microbiological Culture Collection Center at May 27, 2013, and has an accession number of CGMCC No. 7646. The bacterial strain of the invention is screened from excrements of livestocks, and has obvious removing effects on various offensive odor; the average removing rate of NH3 is 97.75%; the average removing rate of H2S is 97.40%; the average removing rate of total hydrocarbons is 99.85%; the average removing rate of odor concentration is 90.46%; and the average removing rate of total bacteria is 71.23%. The biological deodorant of the invention can be used for deodorization and purification of garbage, sewage, and air of livestock and poultry houses.

Description

Technical field [0001] The invention belongs to the field of environmental protection, and relates to a strain of Bacillus coagulans, a preparation method thereof, and application in a biological deodorant. Background technique [0002] With the acceleration of the process of urbanization in our country, the urban population is increasing day by day, followed by a rapid increase in the amount of urban domestic waste. According to incomplete statistics, in recent years, the growth rate of garbage in my country has reached more than 10%, and nearly 150 million tons are generated annually. At present, the domestic urban domestic garbage stock has reached 7 billion tons, covering an area of ​​about 800,000 mu, of which 80% to 90% are from large and medium-sized cities. The average amount of garbage generated per person per day is about 1.2 kg, but its treatment rate Only about 50%. Of the more than 600 cities nationwide, two-thirds of large and medium-sized cities have fallen into ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61L9/01A61L9/012C12R1/07A61L101/52
Inventor 杨继国任杰司卫丽蓝启添
Owner SHENZHEN XINBAO ENVIRONMENTAL PROTECTION ENERGY SCI & TECH CO LTD
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