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Chemiluminescent detection kit for canine distemper virus antigen

A technology for chemiluminescence detection and canine distemper virus, which is applied in measurement devices, scientific instruments, instruments, etc., can solve the problems of high cost, low sensitivity, slow diagnosis, etc., and achieves improved detection efficiency, low test condition requirements, and low cost. low cost effect

Active Publication Date: 2015-12-09
LUOYANG LAIPSON INFORMATION TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of low sensitivity, high cost and slow diagnosis of existing canine distemper virus detection, the present invention provides a canine distemper virus antigen chemiluminescent detection kit

Method used

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  • Chemiluminescent detection kit for canine distemper virus antigen

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Embodiment Construction

[0016] A chemiluminescent detection kit for canine distemper virus antigen, comprising a coating plate and reagents arranged in the box body, the reagents include horseradish peroxidase-labeled goat anti-mouse antibody, coating solution, blocking solution, washing Liquid and chemiluminescent liquid, the coated plate is a luminescent plate coated with canine distemper virus antibody, the dilution of the canine distemper virus antibody is 1:700-1000, horseradish peroxidase-labeled sheep antibody The mouse antibody dilution is 1:2500;

[0017] The luminescent plate is a milky white opaque polystyrene 96-well chemiluminescent plate;

[0018] The coating of the canine distemper virus antibody is to place the canine distemper virus antibody in a 0.1mol / L coating solution, react and coat at a temperature of 4°C for 12h, and then use a blocking solution to seal it on a luminescent plate. inside the hole;

[0019] The coating solution is a carbonate buffer made by dissolving sodium c...

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Abstract

The invention provides a chemiluminiscence detection kit for a canine distemper virus antigen. The chemiluminiscence detection kit comprises a coated plate and a reagent which are arranged in a box body, wherein the reagent comprises a horseradish peroxidase marked goat anti-mouse antibody, a coating solution, a sealing solution, a washing solution and chemiluminiscence liquid; the coated plate is a light-emitting plate coated with a canine distemper virus antibody; the diluting degree of the canine distemper virus antibody is 1:(700-1000); the diluting degree of the horseradish peroxidase marked goat anti-mouse antibody is 1:2500. The chemiluminiscence detection kit for the canine distemper virus antigen has the characteristics of low cost, convenience, rapidness and low testing condition requirements; the detection efficiency is improved and the detection accuracy is also guaranteed; the chemiluminiscence detection kit can be used for export inspection, food export inspection and field rapid detection of an animal breeding industry.

Description

technical field [0001] The invention relates to a kit for virus immunity and detection, in particular to a chemiluminescent detection kit for canine distemper virus antigen. Background technique [0002] Canine distemper (CD) is an acute, highly contagious infectious disease caused by canine distemper virus (CDV) infection in dogs. Canine distemper virus is highly contagious and can cause illness and even death in dogs and some other carnivorous animals. Puppies of 3 to 6 months are more susceptible to canine distemper than adult dogs. Canine distemper virus often causes the death of dogs, so it is very necessary to detect it with a canine distemper virus antigen detection kit in the early stage of similar symptoms. [0003] At present, the traditional detection methods of canine distemper virus include serological detection methods and etiological detection methods. Serological detection methods are mainly hemagglutination test, enzyme-linked immunosorbent assay and immu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569
CPCG01N33/54393G01N33/56983G01N2333/13
Inventor 王善普吴庭才王兴兵王晓军王宇东
Owner LUOYANG LAIPSON INFORMATION TECH
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