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Kit or biological chip for detecting miRNAs for early colonic adenocarcinoma and rectal adenocarcinoma

A detection kit, a technology for rectal adenocarcinoma, applied in the field of medical diagnosis, can solve the problems of sensitivity and specificity

Active Publication Date: 2014-12-10
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the only tumor marker currently recommended clinically for the diagnosis and prognosis of colorectal adenocarcinoma is CEA, and the sensitivity and specificity of CEA as a tumor marker in the diagnosis of colorectal adenocarcinoma has also been questioned.

Method used

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  • Kit or biological chip for detecting miRNAs for early colonic adenocarcinoma and rectal adenocarcinoma
  • Kit or biological chip for detecting miRNAs for early colonic adenocarcinoma and rectal adenocarcinoma
  • Kit or biological chip for detecting miRNAs for early colonic adenocarcinoma and rectal adenocarcinoma

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Embodiment 1

[0046] 1. Research object

[0047] Serum samples included a total of 307 patients with colorectal adenocarcinoma (CAC), 164 patients with colorectal adenoma, and 226 healthy controls. All patients with colorectal adenocarcinoma and adenoma were inpatients in general surgery at Qilu Hospital of Shandong University (hereinafter referred to as our hospital). All were confirmed by postoperative pathological examination. The serum of the healthy control was obtained from the healthy subjects in the Health Examination Center of our hospital, and no obvious abnormalities were found in the physical examination reports. All samples were performed with the consent of the subjects.

[0048] 2. Specimen collection

[0049] Collect 3ml of serum, centrifuge at 1600g for 5 minutes, then centrifuge at 16000g for 10 minutes, separate the supernatant, and store it at -80°C until testing. Mix 3 μl of the isolated supernatant with 3 μl of the RNA isolation solution, centrifuge at 16,000 g for...

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Abstract

The invention discloses a serum miRNAs specific expression profile for early colonic adenocarcinoma and rectal adenocarcinoma, which is formed by up-regulated expressed miR-19a-3p, miR-92a-3p and miR223-3p and down-regulated expressed miR422a. A reverse transcription primer and a detection primer, which are shown as SEQ ID NO: 1-9, of the miRNAs are specifically expressed in the serum miRNAs specific expression profile for the early colonic adenocarcinoma and rectal adenocarcinoma. The invention also provides a serum miRNAs disgnosis model for the early colonic adenocarcinoma and rectal adenocarcinoma, the following formula of logit(P=CAC)=0.4424-0.0092*(miR-19a-3p)-0.0368*(miR-92a-3p)-0.0517*(miR-223-3p)+0.2439*(miR-422a) is used for calculation and for the diagnosis on the early colonic adenocarcinoma and rectal adenocarcinoma, the diagnosis efficiency of the serum miRNAs model on the colonic adenocarcinoma and rectal adenocarcinoma is higher and is higher than that of the traditional tumor marker CEA through ROC (Receiver Operating Characteristic) curve analysis, and the model can used for the diagnosis on the early colonic adenocarcinoma and rectal adenocarcinoma patient (TNM I / II period).

Description

technical field [0001] The invention relates to the specific expression profile of early colorectal adenocarcinoma miRNAs, its reverse transcription primer and detection primer, and a diagnostic model, belonging to the technical field of medical diagnosis. Background technique [0002] Colorectal adenocarcinoma is one of the most common malignant tumors of the digestive system, and its morbidity and mortality are increasing year by year. The prognosis of patients with colorectal adenocarcinoma depends on the stage of the disease at the time of diagnosis. According to statistics, the 5-year survival rate of patients with colorectal adenocarcinoma without invasion and metastasis can be as high as 90%, and the 5-year survival rate of patients with local metastasis is about 68%. %, while the 5-year survival rate of distant metastases is only about 11%. Therefore, early detection of colorectal adenocarcinoma is the key to improving patient prognosis and reducing mortality. [0...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 王传新郑桂喜张欣
Owner SHANDONG UNIV QILU HOSPITAL
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