miRNAs specific expression profile and diagnosis model for early colonic adenocarcinoma and rectal adenocarcinoma
A technology for specific expression profiling and rectal adenocarcinoma, which can be used in the field of early colorectal adenocarcinoma miRNAs specific expression profiling and diagnostic models, which can solve the problems of sensitivity and specificity.
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[0046] 1. Research object
[0047] Serum samples included a total of 307 patients with colorectal adenocarcinoma (CAC), 164 patients with colorectal adenoma, and 226 healthy controls. All patients with colorectal adenocarcinoma and adenoma were inpatients in general surgery at Qilu Hospital of Shandong University (hereinafter referred to as our hospital). All were confirmed by postoperative pathological examination. The serum of the healthy control was obtained from the healthy subjects in the Health Examination Center of our hospital, and no obvious abnormalities were found in the physical examination reports. All samples were performed with the consent of the subjects.
[0048] 2. Specimen collection
[0049] Collect 3ml of serum, centrifuge at 1600g for 5 minutes, then centrifuge at 16000g for 10 minutes, separate the supernatant, and store it at -80°C until testing. Mix 3 μl of the isolated supernatant with 3 μl of the RNA isolation solution, centrifuge at 16,000 g for...
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