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Primer for rapidly detecting drug tolerance of pertussis bordelella erythrocin from specimen and detection method

A pertussis and erythromycin technology, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problems of failure to successfully culture Bordetella pertussis, inability to carry out antibiotic sensitivity tests, and antibiotic sensitivity tests Time-consuming and other problems of development, to achieve the effect of wide application value

Inactive Publication Date: 2014-03-26
西安市疾病预防控制中心
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Problems solved by technology

[0009] In order to solve the technical problems that the antibiotic sensitivity test of Bordetella pertussis in the background technology cannot be successfully cultured, and the antibiotic sensitivity test is time-consuming, the present invention provides a direct and rapid method for detecting Primers and detection method for detection of erythromycin resistance of infected Bordetella pertussis

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  • Primer for rapidly detecting drug tolerance of pertussis bordelella erythrocin from specimen and detection method
  • Primer for rapidly detecting drug tolerance of pertussis bordelella erythrocin from specimen and detection method
  • Primer for rapidly detecting drug tolerance of pertussis bordelella erythrocin from specimen and detection method

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[0036] The invention provides a primer for rapid detection of erythromycin resistance of Bordetella pertussis from samples, and the primer is aimed at the variable regions on both sides of the 2047 site in the 23S rRNA gene of Bordetella pertussis.

[0037] The nucleotide sequences of the primers are:

[0038] 1505F: 5'-GGCACGAGCGAGCAAGTCTC-3', and

[0039] 2118R:5'-TCTGGCGACTCGAGTTCTGC-3'.

[0040] A method for detecting the erythromycin resistance of Bordetella pertussis based on the primers described above, the method comprises the following steps:

[0041] 1) Collect positive samples of Bordetella pertussis nucleic acid:

[0042] 1.1) Collect nasopharyngeal swab samples from clinical suspected cases of pertussis;

[0043] 1.2) Place the nasopharyngeal swab specimens collected in step 1.1) of suspected clinical pertussis cases in 0.5ml normal saline and extract DNA;

[0044] 1.3) Detecting the IS481 gene and ptx-Pr gene of the DNA obtained in step 1.2) by PCR;

[0045]...

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Abstract

The invention relates to a primer for rapidly detecting the drug tolerance of pertussis bordelella erythrocin from a specimen and a detection method. The primer includes variable areas on both sides of a locus 2047 specific to a pertussis bordelella 23S rRNA (ribosomal Robonucleic Acid) gene. According to the primer, the technical problems of incapability of carrying out antibiotic susceptibility test, high time consumption of the antibiotic susceptibility test and the like due to incapability of successfully culturing the pertussis bordelella existing in the prior art are solved.

Description

technical field [0001] The invention belongs to the field of biochemical detection, and relates to a clinical laboratory detection method for pathogenic bacteria, in particular to a method for detecting the erythromycin resistance of Bordetella pertussis from samples without relying on the isolation and cultivation of pathogenic bacteria. Primers and detection methods for rapid detection. technical background [0002] Pertussis is an infectious disease caused by Bordetella pertussis infection, which can be infected by people of all ages. Bordetella pertussis is a Gram-negative bacillus with high nutritional requirements for in vitro culture, and it is an obligate aerobic fastidious bacterium. The isolation and culture rate of Bordetella pertussis reported clinically is extremely low, even less than 10%, and it takes a long time to issue a report (7-10 days); however, in most studies in my country, the reported Bordetella pertussis The bacteria isolation and culture rate was...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11
CPCC12Q1/6869C12Q2531/113C12Q2565/125
Inventor 王增国侯铁军杜全丽刘莹习艳丽李恒新
Owner 西安市疾病预防控制中心
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