Automatic continuous liquid exchange cell culture device

Abstract

A cell culture device is capable of automatically and continuously replacing cell culture fluid and providing the conditions required for the cell culture, thereby realizing the automatic and continuous replacement of cell culture fluid in the culture of cell. The device is characterized by comprising an environmental control system, a continuous culture system and a shell, wherein the shell is composed of a main body structure, a right side cover, a main cover, a cover lock and a hinge; the main cover is mounted at the front surface of the main body structure by virtue of the hinge and locked on the main body structure by virtue of the cover lock; the right side cover is fixed on the right side of the main body structure by screws; the environmental control system consists of a heater, a convection fan, a temperature sensor, a thermal insulation structure, CO2 cylinders, an air delivery pipe, a solenoid valve, CO2 sensors, control circuit boards, a display screen and connecting lines; the continuous culture system comprises culture fluid storage bottles, pipelines, a fluid replacement motor, a fluid replacement pump head, fluid inlet pipes, a cell culture bottle cover, fluid output pipes, cell culture flasks, a sample holder and waste fluid collection bottles.

Description

technical field [0001] The invention relates to a cell culture device used in the fields of cell biology and life science, in particular to a cell culture device with the function of automatically replacing culture fluid. Background technique [0002] In the field of cell biology research and life science research, cell culture is the most basic link. During the cell culture process, the regular and quantitative replacement of the cell culture medium is the most critical operation. At present, the commonly used method of replacing cell culture medium in laboratories is realized by manual operation. The disadvantages of this cell culture replacement method are as follows: [0003] 1. The manual medium change operation is intermittent, and it is impossible to achieve continuous quantitative cell culture medium change during the cell culture process. [0004] 2. Manually changing the medium requires taking the cell sample out of the culture environment for operation, and the...

AI Technical Summary

Problems solved by technology

[0003] 1. Intermittent operation of manually changing the medium, unable to achieve continuous quantitative cell culture medium replacement during the cell culture process

[0004] 2. Manually changing the liquid requires taking the cell sample out of the culture environment for operation, and the impact of environmental changes on the cell sample cannot be ignored

[0005] 3. It is difficult to accurately control the amount of culture medium for manual fluid change

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