Method for direct separation of agarose and agaropectin from agar

A technology for agarose and agar ester, which is applied in the field of separating agarose and agar ester, and achieves the effects of cheap raw materials, simple and practical preparation process, low cost and easy recovery

Active Publication Date: 2014-03-26
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of method that directly separates agarose and agar gel ester from agar, it can obtain agar gel ester simultaneously when preparing agarose, has solved especially in polyethylene glycol (PEG) precipitation method preparation Problems with PEG recovery in the agarose process

Method used

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  • Method for direct separation of agarose and agaropectin from agar
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  • Method for direct separation of agarose and agaropectin from agar

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Embodiment 1

[0020] A kind of method of the present invention directly separates and prepares agarose and agar ester from agar, specifically comprises the steps:

[0021] a. Take 16g agar (646g / cm 2 , 1% gel), prepare an agar solution with a concentration of 1wt%, heat and stir to dissolve into a translucent solution, and place it in a water bath at 60°C for 30 minutes at a constant temperature; wherein, the agar used is derived from Gracilaria, Geliflower or Egunia Marine red algae plants;

[0022] b. Take 100g of PEG to prepare a PEG solution with a concentration of 60wt%, heat and stir in a water bath to dissolve it completely and keep the temperature at 60°C for 30min, wherein the molecular weight of PEG is 2,000;

[0023] c. Quickly mix the agar solution obtained in step a and the PEG solution obtained in step b in a water bath at 60°C and stir continuously. After stirring for 5 minutes, a suspension with white precipitate is obtained. Stop stirring and let it stand for 10 minutes. ...

Embodiment 2

[0034] A kind of method of the present invention directly separates and prepares agarose and agar ester from agar, specifically comprises the steps:

[0035] a. Take 16g agar (646g / cm 2 , 1% gel), prepare an agar solution with a concentration of 3wt%, heat and stir to dissolve into a translucent solution, and place it in a water bath at 90°C for 30 minutes at a constant temperature; wherein, the agar used is derived from Gracilaria, Geliflower or Egunia Marine red algae plants;

[0036] b. Take 80g of PEG to prepare a PEG solution with a concentration of 50wt%, heat and stir in a water bath to completely dissolve and keep the temperature at 90°C for 30min, wherein the molecular weight of PEG is 4,000;

[0037] c. Mix the agar solution obtained in step a with the PEG solution obtained in step b in a water bath and stir continuously. After the stirring time is maintained for 10 minutes, stop stirring to obtain a suspension with a white precipitate. After standing for 10 minutes...

Embodiment 3

[0048] a. Take 16g agar (646g / cm 2 , 1% gel), prepare an agar solution with a concentration of 4wt%, heat and stir to dissolve it into a translucent solution, and place it in a water bath at 80°C for 30 minutes at a constant temperature; wherein, the agar used comes from Gracilaria, Geliflower or Egunia Marine red algae plants;

[0049] b. Take 100g of PEG to prepare a PEG solution with a concentration of 40wt%, heat and stir in a water bath to dissolve it completely and keep the temperature at 80°C for 30min, wherein the molecular weight of PEG is 6,000;

[0050] c. Mix the agar solution obtained in step a with the PEG solution obtained in step b in a water bath and stir continuously. After the stirring time is maintained for 15 minutes, stop stirring to obtain a suspension with a white precipitate. After standing for 25 minutes, a white color appears precipitation;

[0051] d. Take the suspension obtained in step c out of the water bath, let it stand, and cool it at room t...

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Abstract

The invention relates to a method for direct separation and preparation of agarose and agaropectin from agar. The method comprises the following steps: 1, respectively preparing an agar solution and a polyethylene glycol (PEG) solution, at a constant temperature, mixing, stirring, allowing to stand, and generating a precipitate; 2, centrifuging the mixed solution, to obtain the precipitate and a supernatant; 3, washing the precipitate with pure water and anhydrous ethanol, and filtering to obtain a filter cake and a filtrate; 4, drying the filter cake, and smashing to obtain agarose; 5, adding ethanol into the concentrated supernatant, allowing to stand, and thus obtaining a precipitate; 6, centrifuging the mixed solution, and filtering to obtain the precipitate and a filtrate; 7, drying the precipitate to obtain agaropectin; 8, merging the filtrates, concentrating under reduced pressure, collecting fractions, and recycling ethanol; and 9, further concentrating under reduced pressure to a molten state, cooling to solidify, drying, and recycling PEG. The method not only obtains agaropectin during the process of precipitation preparation of agarose, but also solves the problems of recycling the precipitating agent PEG and ethanol, thereby achieving the effects of cyclic utilization and environmental protection.

Description

technical field [0001] The invention relates to a production process for separating agarose and agar ester by using agar as a raw material, which not only obtains high-quality agarose and agar ester simultaneously, but also solves the problem of precipitation agent recovery. technical background [0002] Agar (Agar), as one of the world's three major industrial alginate gels, is widely used in food, biotechnology and medicine, and the performance of agarose (Agarose) isolated from agar is better than that of agar. Agarose is a neutral polysaccharide that does not contain sulfate groups in agar. It is a chain polysaccharide combined with D-galactose and 3,6-anhydro-L-galactose. It is not only widely used in the food industry, but also in advanced biochemistry. The basic substances and important materials of molecular biology, immunology and modern biotechnology, especially in the fields of electrophoresis, chromatography and other biotechnology, neutral agarose is more needed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/12
Inventor 肖美添柯庆勇叶静黄雅燕张学勤赵鹏
Owner HUAQIAO UNIVERSITY
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