Compound drug-carrying liposome as well as preparation method and application thereof
A technology of liposomes and long-circulation liposomes, which is applied in liposome delivery, pharmaceutical formulations, anti-tumor drugs, etc., can solve the problems of poor curative effect, achieve small side effects, high clinical application value, and inhibit chemotherapeutic drug resistance. The effect of drug-induced tumors
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[0031] The invention provides a method for preparing a composite drug-loaded liposome, which is characterized in that the method comprises, using a thin film-ultrasonic method, combining liposome source, distearoylphosphatidylethanolamine-methoxypolyethylene glycol 2000 and pharmaceutical active components to prepare the complex drug-loaded liposome, the pharmaceutical active components are anthracycline antibiotics and resveratrol.
[0032] According to the method of the present invention, in the pharmaceutical active component, the molar ratio of anthracycline antibiotics and resveratrol can be adjusted as required, preferably, the molar ratio of the anthracycline antibiotics and resveratrol is 1:0.01-100, more preferably 1:1-80, more preferably 1:3-40, most preferably 1:5-20.
[0033] In the present invention, the anthracycline antibiotics may be various anthracycline antibiotics commonly used in the art. Since anthracycline antibiotics all have the structural skeleton of ...
Embodiment 1
[0054] This example is used to prepare composite drug-loaded liposome: ADR+Res liposome-1.
[0055] (1) Put 4×10 -2 mol soybean lecithin, 10 -2 mol cholesterol, 2×10 -3 mol distearoylphosphatidylethanolamine-methoxy polyethylene glycol 2000 is mixed with 150ml of absolute ethanol, and after dissolving, add 1.2×10 -3 mol resveratrol to obtain the first mixed solution,
[0056] (2) Evaporate and remove the absolute ethanol in the first mixed solution to form a thin film,
[0057] (3) Put 3×10 -4 mol of anthracycline antibiotics was mixed with 5ml of normal saline, added to the film and sonicated to obtain composite drug-loaded liposomes, ie ADR+Res liposome-1.
[0058] The conditions of the ultrasound include: the frequency is 350W, the ultrasound is 18s, the interval is 8s, and the ultrasound is 15 times.
Embodiment 2
[0060] This example is used to prepare composite drug-loaded liposome: ADR+Res liposome-2.
[0061] (1) Put 4×10 -2 mol soybean lecithin, 10 -2 mol cholesterol, 2×10 -3 mol distearoylphosphatidylethanolamine-methoxy polyethylene glycol 2000 is mixed with 200ml of absolute ethanol, and after dissolving, add 3.6×10 -3 mol resveratrol to obtain the first mixed solution,
[0062] (2) Evaporate and remove the absolute ethanol in the first mixed solution to form a thin film,
[0063] (3) Put 3×10 -4 mol of anthracycline antibiotics was mixed with 4ml of normal saline, added to the film and sonicated to obtain composite drug-loaded liposomes, ie ADR+Res liposome-2.
[0064] The conditions of the ultrasound include: the frequency is 400W, the ultrasound is 15s, the interval is 5s, and the ultrasound is 10 times.
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