Method for preparing mannooligosaccharide from konjac powder

A technology of oligomannose and konjac flour is applied in the field of bioengineering, which can solve the problems of high substrate viscosity, high concentration production, high cost of enzyme production and use, low catalytic efficiency of key enzymes, etc. The effect of low cost, short cycle and high catalytic efficiency

Inactive Publication Date: 2014-03-19
JIANGNAN UNIV
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AI Technical Summary

Problems solved by technology

At present, mannose oligosaccharides are usually prepared by high-temperature hydrolysis, acid hydrolysis or alkaline hydrolysis. Although enzymatic hydrolysis has also been reported, the conditions are not mature enough.
The enzymatic production of mannose oligosaccharides is mainly limited by the low catalytic efficiency of the key enzymes used in the production, the high cost of enzyme production and use, and the high viscosity of the substrate, which makes it difficult to achieve high-concentration production, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 3

[0025] Embodiment 3 double enzymatic method prepares the mannose oligosaccharide in konjac fine powder

[0026] 30g·L prepared with deionized water under optimized conditions -1 Konjac gum solution as substrate, 60U·g -1 reAuMan5A of konjac powder N3C3 And supplemented with reAuCell2A of different units of enzyme activity (enzyme activity is measured with 0.5% sodium carboxymethylcellulose as a substrate) at 60 ° C for 6 hours, then use the DNS method to measure the content of reducing sugar in the hydrolyzate and calculate konjac essence powder hydrolysis rate. The results showed that reAuCell2A can significantly promote reAuMan5A N3C3 The hydrolysis of konjac fine powder greatly improves the hydrolysis rate of konjac fine powder, and the hydrolysis rate can reach 65.36%.

[0027] Qualitative analysis of embodiment 4 konjac powder hydrolyzate

[0028] In the present invention, a silica gel plate is used as a chromatographic carrier, a 2:1:1 mixture of butanol, glacial ac...

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PUM

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Abstract

The invention provides a method for preparing mannooligosaccharide from konjac powder. The mannooligosaccharide is obtained by utilizing the synergistic effect of recombinant beta-mannanase (reAuMan5AN3C3) and recombinant beta-endoglucanase (reAuMan5AN3C3) from a laboratory to perform enzymolysis on the konjac powder to obtain mannooligosaccharide. The catalytic efficiency of a key enzyme used by the method is relatively high, the production and using cost of the enzyme is relatively low, the period is short, the pollution is avoided, and the method has relatively great industrial production and application potential and economic values.

Description

technical field [0001] The invention relates to a method for preparing mannose oligosaccharides by hydrolyzing konjak flour with biological enzymes, and belongs to the technical field of bioengineering. Background technique [0002] Mannooligosaccharide (Mannooligosaccharide, MOS) is an oligosaccharide polymerized by 2 to 10 mannose through β-1,4 glycosidic bonds, also known as mannooligosaccharide. It can effectively promote the proliferation of intestinal probiotics represented by bifidobacteria in organisms, and has the functions of inhibiting the growth of pathogenic bacteria in the body, reducing the production of toxic metabolites, preventing constipation, protecting the liver, anti-tumor and enhancing the body's immunity, etc. Physiological function, is a new generation of functional food. my country has formulated functional oligosaccharide industry standards and listed mannose oligosaccharides as one of them, but the market share of corresponding domestic products ...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/12C12P19/04C12P19/00
Inventor 李剑芳陆源赵梅唐存多邬敏辰
Owner JIANGNAN UNIV
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