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Stable gel containing bromelain

A bromelain and proteolytic enzyme technology, applied in the field of medicine, can solve the problems of high humidity and temperature sensitivity, inconvenient use, etc.

Active Publication Date: 2014-03-19
NKD PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, because the raw material of medicinal bromelain is sensitive to high humidity and temperature, it will degrade and inactivate quickly in aqueous solution or in a state with more water, so the dosage forms provided are limited to capsules, powders, etc., which need to be prepared with solvents before use. Inconvenient to use

Method used

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  • Stable gel containing bromelain
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] The refining process of bromelain: 1) Take a certain amount of bromelain, add 15 times of pH 7.2, 0.05M phosphate buffer for extraction, stir magnetically for 4 hours at 4-10°C, and centrifuge at 10,000 rpm for 10 minutes , collect the supernatant; or remove insoluble impurities in the leachate by filtration;

[0078] 2) Precipitate the collected liquid with ammonium sulfate with 45% saturation, after precipitation, centrifuge to remove the supernatant;

[0079] 3) The precipitate was dialyzed with distilled water for desalination (1:100), and ammonium sulfate was removed to obtain a concentrated solution.

[0080] 4) Further freeze-drying to obtain refined bromelain; or the concentrated solution is precipitated with cold acetone and centrifuged with a refrigerated centrifuge to obtain a precipitate, which can be naturally dried at low temperature to obtain refined bromelain.

[0081] The pineapple proteolytic enzyme refined product obtained by the above method has a h...

Embodiment 2

[0083] The refining process of bromelain: 1) Take a certain amount of bromelain, add 20 times of pH 6.0, 0.02M phosphate buffer for extraction, stir magnetically for 2 hours at 4-10°C, and centrifuge at 4000 rpm for 10 minutes , collect the supernatant; or remove insoluble impurities in the leachate by filtration;

[0084] 2) Precipitate the collected liquid with ammonium sulfate with 45% saturation, after precipitation, centrifuge to remove the supernatant;

[0085] 3) The precipitate was dialyzed with distilled water for desalination (1:100), and ammonium sulfate was removed to obtain a concentrated solution.

[0086]4) Further freeze-drying to obtain refined bromelain; or the concentrated solution is precipitated with cold acetone and centrifuged with a refrigerated centrifuge to obtain a precipitate, which can be naturally dried at low temperature to obtain refined bromelain.

[0087] The pineapple proteolytic enzyme refined product obtained by the above method has a hydr...

Embodiment 3

[0088] Example 3: PLGA-PEG-PLGA

[0089] 1) Weigh 6g of mixed lactide, 1g of glycolide, and 15003g of polyethylene glycol;

[0090] 2) Place mixed lactide and glycolide in an oven at 40°C for 2 hours, and put polyethylene glycol 1500 in an oven at 140°C for 2 hours;

[0091] 3) Mix the swirling lactide, glycolide and polyethylene glycol 1500 after waving into the same vial and add the catalyst stannous isooctanoate (0.1%, w / w), vacuum seal and press the cap ;

[0092] 4) Put the capped vial in an oven at 140°C to react for 12 hours. After completion, pour it out while it is hot, cool it down to room temperature naturally, dissolve it in purified water at 4°C, and heat it to 70°C after complete dissolution to produce precipitation. Discard the water layer, then add purified water at 4°C, and repeat the operation 3 times to remove the reacted monomer compound and low molecular weight water-soluble copolymer.

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Abstract

The invention relates to stable gel containing bromelain. The stable gel contains bromelain as an active ingredient, and a pharmaceutical acceptable carrier PLGA-PEG-PLGA, and has the characteristics of being good in stability, convenient to use and quick to act, simultaneously has bacteriostatic action, is applied to protein denaturation repair, inflammation diminishing, pain relieving, and antibiotic effect improvement, and belongs to the technical field of medicines.

Description

technical field [0001] The invention relates to a bromelain-containing gel, which has the characteristics of good stability, convenient use, and rapid action, and has antibacterial effect at the same time, and is used for protein denaturation repair, anti-inflammation, pain relief, and increasing the effect of antibiotics. field of medical technology. Background technique [0002] Bromelain (Bromelain, referred to as bromelain, also known as bromelain or bromelain) is a pure natural product extracted from pineapple stems, leaves, and skins, refined, purified, concentrated, enzyme immobilized, and freeze-dried. plant protease. Its appearance is light gray powder, the molecular weight is 33000, the isoelectric point is 9.55, and the optimum pH value is 6-8. Soluble in water, insoluble in ethanol, chloroform and ether. Is a glycoprotein. The main principle of action: to hydrolyze polypeptides into low molecular weight peptides. It also has the function of hydrolyzing amide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/34A61K38/48A61P31/04A61P29/00A61P17/02A61P17/00
Inventor 尚丽霞
Owner NKD PHARMA CO LTD
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