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Application of Renqing Changjue in the preparation of drugs for treating ovarian cancer

A technology of ovarian cancer and common sense, applied in the field of medicine, can solve problems such as threats to women's lives

Active Publication Date: 2016-03-30
JINKE TIBETAN MEDICINE QINGHAI PROV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the number of deaths due to ovarian cancer ranks first among all types of gynecological tumors, posing a serious threat to women's lives

Method used

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  • Application of Renqing Changjue in the preparation of drugs for treating ovarian cancer
  • Application of Renqing Changjue in the preparation of drugs for treating ovarian cancer
  • Application of Renqing Changjue in the preparation of drugs for treating ovarian cancer

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0023] Experimental example 1, preparation of each extract of Renqing Changjue

[0024] 1. Samples

[0025] Renqing Changjue, produced by Qinghai Jinhe Tibetan Medicine Co., Ltd., batch number: 20100707

[0026] 2. Methods and steps

[0027] 1. Petroleum ether extract: Take 50g of finely ground Renqing Changjue powder, and extract by boiling 6 times its weight in petroleum ether (60°C-90°C) for 3 times, 1 hour each time. Recover petroleum ether under reduced pressure at 40°C-50°C to obtain 2.71 g of petroleum ether extract.

[0028] 2. Ethyl acetate extract: take 50 g of the finely ground fine powder of Renqing Changjue, and extract it by boiling with ethyl acetate 6 times its weight for 3 times, each time for 1 hour. Ethyl acetate was recovered under reduced pressure at 50°C-60°C to obtain 7.66 g of ethyl acetate extract.

[0029] 3. Ethanol extract: Take 50g of fine powder of Renqing Changjue after fine grinding, extract 3 times with 8 times the volume of ethanol of its ...

experiment example 2

[0031] Experimental example 2, the inhibitory effect of Renqing Changjue on ovarian cancer

[0032] 1. Experimental principle

[0033] Succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-purple crystalline formazan (Formazan) and deposit it in the cells. The amount of formazan crystals produced is only proportional to the number of living cells (dead The succinate dehydrogenase in the cells disappears, and MTT cannot be reduced). Formazan crystals generated by reduction can be dissolved in MTT solution containing 50% N,N-dimethylformamide and 20% sodium dodecylsulfonate (pH4.7), and measured at 490nm by a microplate reader The optical density at OD value reflects the number of living cells.

[0034] 2. Drugs and reagents

[0035] 1. Animals

[0036] 90 healthy Kunming mice of SPF grade, weighing 30±2g, were provided by Shandong Lukang Pharmaceutical Co., Ltd. New Experimental Animals, animal qualification certificate...

Embodiment 1

[0079] Renqing Changjue, 1g / pill, 1 pill a day, take 1 gram (1 pill) every 3-7 days; grind it and take it on an empty stomach at dawn.

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PUM

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Abstract

The invention relates to the application of Renqing Changjue in the preparation of drugs for treating ovarian cancer. The Renqing Changjue is a concentrated pill or a sugar-coated tablet or a film-coated tablet or capsule made by preparing the active ingredient from its prescription medicinal materials. The active ingredient is one or more of petroleum ether extract, ethyl acetate extract, ethanol extract and water extract. The present invention overcomes the technical bias in the application of the prior art, and finds through pharmacological experiments that various extracts of Renqing Changjue have better inhibitory effect on ovarian cancer cells in vitro, and its effect is equivalent to or slightly better than that of cisplatin; The drug-containing serum of various extracts of Qingchangjue also has a significant inhibitory effect on ovarian cancer in vitro (P<0.05), and the effects of petroleum ether extract, ethyl acetate extract and water extract are slightly better than those of cisternone. platinum.

Description

technical field [0001] The invention relates to the application of Renqing Changjue in the preparation of drugs for treating ovarian cancer, and belongs to the field of medicine. Background technique [0002] Ovarian cancer is one of the most common tumors in women, ranking third in incidence after cervical cancer and uterine body cancer. However, the number of deaths due to ovarian cancer ranks first in all types of gynecological tumors, posing a serious threat to women's lives. The etiology of ovarian cancer is still unclear, and its incidence may be related to age, fertility, blood type, mental factors and environment. [0003] Renqing Changjue is a Tibetan proven prescription. It was formulated in the eighth century. It was first used as a special medicine for Tibetan kings, Panchen Lamas, Dalai Lamas, and dignitaries. It has been used for more than a thousand years. Now, Renqing Changjue is recorded in " Chinese Pharmacopoeia (2010 edition). Renqing Changjue is compo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/88A61P35/00A61K33/28A61K35/32A61K35/413A61K35/618
Inventor 张义智单玉刚付素心彭坤马宁
Owner JINKE TIBETAN MEDICINE QINGHAI PROV
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