Construction method of in vitro regeneration system of tea tree

A technology of in vitro regeneration and construction method, which is applied in the field of tea tree in vitro regeneration system construction, can solve the problems of small comparability, long cultivation period, poor stability, etc., and achieve the goal of maintaining genetic stability, short cultivation period and stable method Effect

Active Publication Date: 2014-03-05
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some problems in tea tree tissue culture, which greatly restrict the application of tissue culture technology in tea trees. When the adventitious buds or axillary buds are used as explants, the shoot rate is low, the growth is slow, and the culture period is long. Rootless tea seedlings are relatively difficult to root, and the survival rate of transplanted field is low; ② For different tea varieties, the results of tissue culture are different. Large, low comparability, even the tissue culture results of the same tea tree variety are difficult to repeat, poor stability, unable to form a complete and reliable method system; ③ Genetic engineering has become a powerful means of plant genetic improvement, the success or failure of genetic manipulation Depends on the regeneration of isolated plants, but the regeneration technology of tea trees is not mature enough to establish a high-frequency, stable regeneration system

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for establishing a tea tree in vitro regeneration system, specifically comprising steps as follows:

[0017] 1) After sterilizing the tea tree stems with full axillary buds, culture them on MS + 1.5 mg / L 6-BA + 0.3 mg / L NAA medium for 30 days;

[0018] 2) When the buds grow to 3 cm, cut them into 1 cm long stems and insert them into MS+1.0mg / L 6-BA+0.1mg / LNAA medium for proliferation;

[0019] 3) When the proliferated sprouts grow to 4 leaves, carry out rooting culture on 1 / 2 MS+0.2 mg / L NAA medium;

[0020] 4) When the roots of the tissue-cultured seedlings cultivated by rooting grow to 5cm, with an average of 5 roots, the hardened seedlings are transplanted. After the tissue-cultured seedlings are transplanted to the outdoor culture for 30 days, the survival rate of the plants is 67%, and the growth status of the plants Better, the leaves are emerald green.

Embodiment 2

[0022] A method for establishing a tea tree in vitro regeneration system, specifically comprising steps as follows:

[0023] 1) After sterilizing the tea tree stems with full axillary buds, culture them on MS + 1.5 mg / L 6-BA + 0.3 mg / L NAA medium for 32 days;

[0024] 2) When the buds grow to 4 cm, cut them into 1 cm long stems and insert them into MS+1.0mg / L 6-BA+0.1mg / LNAA medium for proliferation;

[0025] 3) When the proliferated sprouts grow to 5 leaves, carry out rooting culture on 1 / 2 MS+0.2 mg / L NAA medium;

[0026] 4) When the roots of the tissue-cultured seedlings cultivated by rooting grow to 5.4 cm, with an average of 5 roots, the hardened seedlings are transplanted. After the tissue culture seedlings were transplanted to outdoor for 30 days, the survival rate of the plants was 65%, and the growth of the plants was better, and the leaves were emerald green.

Embodiment 3

[0028] A method for establishing a tea tree in vitro regeneration system, specifically comprising steps as follows:

[0029] 1) After sterilizing the tea tree stems with full axillary buds, culture them on MS + 1.5 mg / L 6-BA + 0.3 mg / L NAA medium for 35 days;

[0030] 2) When the bud grows to 5cm, cut it into a 1.5cm-long stem segment and insert it into MS+1.0mg / L 6-BA+0.1mg / LNAA medium for proliferation;

[0031] 3) When the proliferated sprouts grow to 4 leaves, carry out rooting culture on 1 / 2 MS+0.2 mg / L NAA medium;

[0032] 4) When the roots of the tissue-cultured seedlings cultivated by rooting grow to 6 cm, with an average of 4 roots, the hardened seedlings are transplanted. After the tissue culture seedlings were transplanted to the outdoor for 30 days, the survival rate of the plants was 68%, and the growth conditions of the plants were better, and the leaves were emerald green.

[0033] Utilizing the method of the present invention to construct a tea tree in vitro ...

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Abstract

The invention discloses a construction method of an in vitro regeneration system of a tea tree. According to the construction method, the construction of the in vitro regeneration system takes a stem with an axillary bud as an explant, the stem is cultured, the axillary bud is germinated to grow leaves upwards and roots downwards, and then a seedling is directly formed without callus induction. According to the invention, the stem of Fuding Dabaicha is used as the explant and the construction method of the regeneration system is researched; the hereditary stability of tea tree varieties is kept and the storage of germplasm resources of tea trees can also be promoted.

Description

technical field [0001] The invention belongs to the technical field of tea tree tissue culture, and in particular relates to a method for constructing a tea tree in vitro regeneration system. Background technique [0002] tea tree( Camellia sinensis L. Kuntze.) belongs to Theaceae, Camellia, is a perennial woody, evergreen plant. Tea tree tissue culture began in the 1960s. The research on tea tree tissue culture in my country started relatively late, but in recent years, with the joint efforts of scientific researchers, significant progress has been made. Tea tree seeds, stems, Axillary buds, anthers, etc. were cultured to obtain complete regenerated plants. However, there are some problems in tea tree tissue culture, which greatly restrict the application of tissue culture technology in tea trees. When the adventitious buds or axillary buds are used as explants, the shoot rate is low, the growth is slow, and the culture period is long. Rootless tea seedlings are relative...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 孙威江吴婉婉陈志丹何碧珠
Owner FUJIAN AGRI & FORESTRY UNIV
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