Method for detecting resistance of cotton plants to greensickness by molecular marker-assisted selection

Abstract

The invention provides a method for detecting the resistance of cotton plants to greensickness by molecular marker-assisted selection. The method comprises the following steps: (1), soaking seeds of cotton plants to be detected in a soaking solution; (2), planting the soaked seeds in a disease garden; (3), selecting a cotton plant capable of surviving in the disease garden as a preliminary screened cotton plant; and (4), respectively carrying out PCR (Polymerase Chain Reaction) amplification on genome DNA (Desoxvribose Nucleic Acid) of the cotton plant by a first primer pair and a second primer pair to obtain a first amplification product and a second amplification product, wherein the first primer pair is shown as SEQ ID NO:1 and SEQ ID NO:2, the second primer pair is shown as SEQ ID NO:3 and SEQ ID NO:4, and if the first amplification product has nucleic acid shown as SEQ ID NO:5 and the second amplification product has nucleic acid shown as SEQ ID NO:6, the cotton plant to be detected is a greensickness-resistant plant. According to the method, the resistance to greensickness is predicted, the cotton plants are screened to discard plants which do not show symptoms but are susceptible in a disease garden screening process, the waste of manpower and material resources is reduced, and the breeding efficiency is increased.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a method for detecting the resistance of cotton plants to Verticillium wilt. Background technique [0002] Cotton verticillium wilt is a major plant disease worldwide, causing 10-15% yield loss to cotton production all the year round, and more than 60% of the country's cotton planting area in serious years, causing a heavy blow to cotton production. The pathogenic bacteria spread along with the seeds, broken branches and leaves, and the soil. The sclerotia can survive in the soil for many years, and chemical drugs, cultivation and other methods are ineffective against it. It is called the "cancer" of cotton by the phytopathological circle, and it has become a long-standing unsolved problem in the world. sexual conundrum. Breeding disease-resistant varieties is recognized as the most economical and effective means to solve cotton Verticillium wilt. The reason why it has ...

AI Technical Summary

Problems solved by technology

After the 1970s, the occurrence of Fusarium wilt of cotton gradually increased, and breeding for resistance to Fusarium wilt began. However, the breeding technology basically used natural disease-susceptible test fields for disease resistance identification. The identification results of hybrid offspring materials are inaccurate, and the selection or elimination of materials is relatively blind

After the 1990s, the occurrence of Verticillium wilt became more and more serious, and there was an urgent need to breed varieties resistant to Verticillium wilt. However, methods similar to those for resistance to Fusarium wilt were still used in the identification and selection of disease resistance. The breeding efficiency was low, and the selection of offspring materials was inaccurate. Poor disease resistance and lower yields of resistant varieties

[0004] Cotton Verticillium wilt resistance molecular marker-assisted breeding technology has been applied in fiber variety improvement, sterile line improvement, etc., but due to the lack of stable, reliable and closely linked molecular markers in the breeding of Verticillium wilt-resistant varieties, it has not been applied to actual production.

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