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One strain of streptomyceslavendulae X33 and applications thereof

A technology of Streptomyces lavender, CCTCCNO.M2013163, applied in the direction of application, bacteria, fungicides, etc., to achieve the effect of simple use, low cost, and remarkable control effect

Inactive Publication Date: 2014-02-26
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are no reports of Streptomyces lilacaceae being applied to green mold, acid rot, tree canker and dieback of fruits and vegetables.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Screening and Identification of Bacterial Strain X33

[0016] Through the antibacterial test in the dish, Penicillium digitatum was used as the indicator bacteria to isolate actinomycetes from the root soil of citrus, and the strain X33 had the strongest antibacterial activity. The antimicrobial spectrum of strain X33 was measured and found that its sterile fermentation filtrate had significant inhibitory effect on 6 kinds of bacteria, 4 kinds of yeast and 6 kinds of phytopathogenic fungi, and the strain X33 had good antibacterial stability in subculture. Micromorphological, physiological and biochemical characteristics, and 16SrDNA sequence analysis, etc., identified the strain X33 as Streptomyces lavender ( Streptomyces lavendulae ).

Embodiment 2

[0017] Embodiment two: 250mL shake flask fermentation culture of bacterial strain X33

[0018] Streptomyces lilacinus griseus X33 was inoculated on Gaoshi No. 1 slant medium, and after the slant spores matured, they were inoculated in a 250mL Erlenmeyer flask containing 30mL seed culture solution at 5-10% of the inoculation amount, at 32°C and 200r / min. Under the shaking culture for 48h. Inoculate the cultivated seed solution into a 250mL Erlenmeyer flask containing 30mL of fermentation medium according to the inoculum amount of 5-10%, and first shake and cultivate at 32°C and 200r / min for 48h, and then at 28°C and 180r / min. Incubate with shaking for 24 hours.

[0019] Various media formulations are as follows:

[0020] Slant medium: soluble starch 2%, KNO 3 0.1%, NaCl0.05%, MgSO 4 0.05%, K 2 HPO 4 0.05%, FeSO 4 ·7H 2 O0.001%, agar 2%, pH7.2-7.4.

[0021] Seed medium: 2% soluble starch, 0.3% beef extract, 0.5% peptone, KNO 3 0.1%, K 2 HPO 4 0.05%, initial pH7.0.

...

Embodiment 3

[0023] Embodiment three: the 50L fermentation culture of Streptomyces lilacaceae X33 bacterial strain

[0024] Inclined surface and seed culture mode are the same as embodiment two. The 50L fermentation tank inoculum volume is 5~10%, the fermentation temperature is 30℃, the tank pressure is 0.05Mpa, the stirring speed is 300r / min, the aeration ratio is 0.6-1.2vvm, the dissolved oxygen is controlled at 10-80%, and the pH of the fermentation broth is maintained at 6.5- Between 7.5 and 72 hours of fermentation time. Determination of the titer of the fermentation broth.

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PUM

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Abstract

The invention discloses a strain of streptomyceslavendulae X33, and the preservation number is CCTCC No. M2013163. The bacterial strain and the fermentation liquid thereof can be used to prevent and treat vegetable penicillium digitatum, penicilliosis, sour rot disease, and canker disease and shoot blight of trees caused by botryosphaeriaspp. The streptomyceslavendulae X33 has the advantages of no toxic or side effect, simple preparation technology, low cost, and user-friendliness, is capable of preventing and treating various plant diseases at the same time, and has a prominent preventing and treating effect.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a strain of Streptomyces lilacinus griseus ( Streptomyces lavendulae )X33 and its application. Background technique [0002] With the growing demand for pollution-free, pollution-free green food and environmental protection, biological control has become another important control method after agricultural chemical control. Many biological fungicides have been published and widely used in production. Significant effect. Streptomyces can produce a variety of secondary metabolites, which play a huge role in plant protection. There are many types of antibiotics produced by it, and most of them have been widely used in agricultural production to prevent and control fungi, bacteria, nematodes and some important diseases. Pests, and some have herbicidal activity. Streptomyces lavendulae is an important antibiotic-producing bacterium. There are many reports on the app...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/56
Inventor 吴晓玉张宇坤段栓成陈金
Owner JIANGXI AGRICULTURAL UNIVERSITY
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