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Preparation method and use of poultry vaccine-specific pig spleen transfer factor

A transfer factor and specific technology, applied in the field of poultry vaccine specific porcine spleen transfer factor preparation, can solve the problem of inability to directly dilute poultry attenuated vaccine, TF is not suitable for poultry vaccine diluent and immune adjuvant, lack of osmotic pressure adjustment process, etc. problems, to achieve the effect of reducing the number of freeze-drying operations, reducing the volume of freeze-drying, and reducing production costs

Active Publication Date: 2014-02-26
派生特(福州)生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] Due to the selection of formaldehyde inactivation and the lack of osmotic pressure adjustment process in the original method of preparing TF, the prepared TF is not suitable for use as a diluent and immune adjuvant of poultry vaccines, that is, it cannot directly dilute poultry attenuated vaccines, and cannot be used with Avian attenuated vaccine virus solution mixed and freeze-dried

Method used

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  • Preparation method and use of poultry vaccine-specific pig spleen transfer factor
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  • Preparation method and use of poultry vaccine-specific pig spleen transfer factor

Examples

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preparation example Construction

[0069] A preparation method for poultry vaccine-specific pig spleen transfer factor, which comprises the following steps:

[0070] 1) Feed healthy pigs with white lentils at a dosage of 30-60g per pig per day for 15-30 days, and then immunize healthy pigs fed with white lentils with more than one poultry vaccine, each poultry vaccine is immunized twice Above, the time interval between two adjacent immunizations is more than 7 days, and 14-28 days after the last poultry vaccine immunization, detect poultry vaccine antibodies, select poultry vaccine antibody-positive pigs for slaughter, and harvest pig spleens;

[0071] 2) Low-temperature preservation: the harvested pig spleen is immediately stored below -40°C, and the storage time does not exceed 20 days;

[0072] 3) Thawing pig spleen: thaw the low-temperature preserved pig spleen through running water. The thawing temperature is lower than 37°C. After thawing, wash it with purified water for 3-5 times, and discard the hardene...

Embodiment 1

[0143] A preparation method for poultry vaccine-specific pig spleen transfer factor, which comprises the following steps:

[0144] 1) Feed healthy pigs with white lentils at a dosage of 30g per pig per day for 18 days, and then immunize the healthy pigs fed with white lentils with bird flu vaccine for more than 2 times, and the time of 2 adjacent immunizations with bird flu vaccine The interval is 7 days, and 14 days after the last immunization with bird flu vaccine, the bird flu vaccine antibody is detected, the pigs with positive bird flu antibody are selected for slaughter, and the pig spleen is harvested;

[0145] 2) Low-temperature preservation: the harvested pig spleen is immediately stored at -40°C, and the storage time does not exceed 20 days;

[0146] 3) Thawing pig spleen: thaw the cryopreserved pig spleen through running water, the thawing temperature is lower than 37°C, wash 3 times with purified water after thawing, and discard the hardened and necrotic pig spleen; ...

Embodiment 2

[0171] A preparation method for poultry vaccine-specific pig spleen transfer factor, which comprises the following steps:

[0172] 1) Feed healthy pigs with white lentils, the dosage is 50g per pig per day, and feed for 21 days, and then immunize the healthy pigs fed with white lentils with chicken infectious bronchitis vaccine and chicken infectious bursal disease vaccine sequentially, Each poultry vaccine is immunized more than twice, and the interval between two adjacent immunizations is 10 days. 20 days after the last poultry vaccine immunization, the poultry vaccine antibody is tested, and the chicken infectious bronchitis or chicken infectious bursitis antibody is positive. Slaughtering pigs, harvesting pig spleens;

[0173] 2) Low-temperature preservation: the harvested pig spleen is immediately stored at -80°C, and the storage time does not exceed 20 days;

[0174] 3) Thawing pig spleen: thaw the cryopreserved pig spleen through running water, the thawing temperature ...

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Abstract

The present invention discloses a preparation method for a poultry vaccine-specific pig spleen transfer factor. The preparation method comprises: selecting poultry vaccine antibody-positive pigs, and slaughtering to obtain pig spleen; carrying out cold preservation; thawing the pig spleen; removing fascia; mincing; homogenizing; filling into a bottle, and storing; carrying out repeated freezing and thawing; carrying out centrifugation; carrying out microfiltration; carrying out ultra-filtration; inactivating; adjusting the pH value; adjusting an osmotic pressure; sterilizing; and testing quality. According to the present invention, in the homogenizing step, the pH value of water for injection is adjusted to 4-6 so as to easily increase yields of ribose and polypeptides; the box type member package tangential flow filtration is adopted, such that the residual dead volume of the system is small, and linear enlargement production is easily achieved; the transfer factor crude product is subjected to tangential flow nano-filtration through a 1-3 KD box type member package to prepare the high concentration transfer factor so as to increase a use effect; phenol red is adopted as a pH value indicator, such that customers easily observe the pH value of the transfer factor; and beta-propiolactone is adopted to replace the traditional formaldehyde to inactivate the transfer factor, and the osmotic pressure adjustment process is added so as to easily achieve transfer factor and vaccine combined immunization.

Description

technical field [0001] The invention belongs to transfer factor, and in particular relates to a preparation method and application of poultry vaccine-specific pig spleen transfer factor. Background technique [0002] Transfer Factor (TF) is a substance released by T lymphocytes that can transfer sensitization information. It can specifically transfer the donor's cellular immune information to the recipient, thereby enhancing the immune function of the recipient. Since the discovery of TF in the 1950s, scholars at home and abroad have conducted a lot of research on it, and introduced it into veterinary immunity and clinical treatment in 1979. At present, TF has gradually become a widely used immune enhancer. [0003] TF has no species specificity, so TF has a wide range of sources. Human TF, pig TF, cattle TF, sheep TF, goose TF, monkey TF, etc. are common in human clinical practice, while bovine TF and pig TF are common in livestock and poultry breeding. Chicken TF. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K35/28A61K47/42A61P37/04A61P31/00
Inventor 徐磊
Owner 派生特(福州)生物科技有限公司
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