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Wax myrtle leaf total-flavonoid extracting process and extracting condition optimization method thereof

An extraction process and a technology of total flavonoids, applied in the field of medicine, can solve the problems of multiple extraction times and long extraction time, and achieve the effect of optimizing the extraction process and good use value

Inactive Publication Date: 2014-02-26
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the existing extraction method of total flavonoids from bayberry leaves is as follows: leaching with ethanol solution, combined with ultrasonic-assisted method, soaking in 40% ethanol 45 times the sample weight for 24 h, ultrasonic-assisted extraction for 45 min, and continuous extraction twice; Ultrasonic-microwave synergistic extraction, the extraction temperature is 70°C, the liquid-to-solid ratio is 25 mL / g, the ethanol concentration is 60%, the extraction time is 40 min, and the extraction times are 2 times; the extraction time is long and the extraction times are many

Method used

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  • Wax myrtle leaf total-flavonoid extracting process and extracting condition optimization method thereof
  • Wax myrtle leaf total-flavonoid extracting process and extracting condition optimization method thereof
  • Wax myrtle leaf total-flavonoid extracting process and extracting condition optimization method thereof

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Experimental program
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Effect test

Embodiment 1

[0061] Fresh bayberry leaves were dried, crushed, passed through a 100-mesh sieve, soaked in 10 times the volume of petroleum ether, degreased, filtered by suction, dried in the shade, and used as sample powder; 0.7 g of sample powder was mixed with 14 mL of ethanol (80%) (that is, liquid material Ratio 20:1), microwave-assisted extraction at 73.3°C for 7.00 min, centrifuged at 3500 r / min for 5 min, and the supernatant was taken to obtain the extract. Diluted to a certain multiple, the obtained extract was tested for antioxidant activity by DPPH method and ABTS method, and then modified AlCl 3 The content of total flavonoids was measured by method, and the content of total flavonoids obtained was 70.94 mg / g.

Embodiment 2

[0063] Fresh red bayberry leaves were dried, crushed, passed through a 100-mesh sieve, soaked in 10 times the volume of petroleum ether, degreased, filtered by suction, dried in the shade, and used as sample powder; 0.7 g of sample powder was mixed with 10.5 mL of ethanol (80%) (that is, liquid material Ratio 15:1), microwave-assisted extraction at 60°C for 7.00 min, centrifuged at 3500 r / min for 5 min, and the supernatant was taken to obtain the extract. Diluted to a certain multiple, the obtained extract was tested for antioxidant activity by DPPH method and ABTS method, and then modified AlCl 3 The content of total flavonoids was measured by method, and the content of total flavonoids obtained was 65.41 mg / g.

Embodiment 3

[0065] Fresh red bayberry leaves were dried, crushed, passed through a 100-mesh sieve, soaked in 10 times the volume of petroleum ether, degreased, filtered by suction, dried in the shade, and used as sample powder; 0.7 g of sample powder was mixed with 7 mL of ethanol (80%) (that is, liquid material Ratio 10:1), microwave-assisted extraction at 80°C for 9.00 min, centrifuged at 3500 r / min for 5 min, and the supernatant was taken to obtain the extract. Diluted to a certain multiple, the obtained extract was tested for antioxidant activity by DPPH method and ABTS method, and then modified AlCl 3 The content of total flavonoids was measured by method, and the content of total flavonoids obtained was 64.06 mg / g.

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Abstract

The invention discloses a wax myrtle leaf total-flavonoid extracting method which takes oxidation activity resistance as an index. The method comprises the steps of mixing ethanol and wax myrtle leaves according to the liquid / material ratio of (10-25)mL: 1g, extracting for 5-11 minutes at the temperature of 60-90 DEG C by adopting a microwave-assisted extraction method, carrying out centrifugation for 5 minutes at the rate of 3,500r / min, and extracting supernatant, thereby obtaining a wax myrtle leaf extract, wherein the extraction time is 7.00 minutes when the extraction temperature is 73.3 DEG C, the equivalent of Trolox in the extract assayed by using a DPPH (2,2-di-phenyl-1-picrylhydrazyl) method and an ABTS (2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid)-diammonium salt) method respectively is 1,352.10mu mol / g and 1,438.73mu mol / g when the liquid / material ratio is 20mL: 1g, and the content of total flavonoids of the extract is 70.94 mg / g. The invention further provides a method for optimizing a wax myrtle leaf total-flavonoid extracting process by using a response surface methodology, wherein oxidation activity resistance is taken as an index, three variables, namely extraction temperature, extraction time and liquid / material ratio, are set, and optimization is carried out by using Design Expert software according to Box-Behnken design principles. The method has the advantages that the cost is low, the extraction time is short, the extraction efficiency is high, and the source of raw materials is wide.

Description

[0001] technical field [0002] The invention belongs to the technical field of medicine, and relates to an extraction process for extracting total flavonoids from bayberry leaves and a method for optimizing extraction conditions thereof, in particular to a method for optimizing extraction conditions of total flavonoids from bayberry leaves by using the response surface method with antioxidant activity as an index. Background technique [0003] Free radicals, an active group or molecule with unpaired electrons produced by the human body during life activities, are intermediate products of normal biochemical processes and exist in both normal and pathological processes of the body. [0004] Free radicals in living organisms mainly include superoxide anion radicals (O 2- ·), hydroxyl radicals (·OH) and lipid peroxidation free radicals (ROO·), etc., can attack biomacromolecules in the body, such as nucleic acids, proteins, polyunsaturated fatty acids, thereby causing toxicity t...

Claims

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Application Information

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IPC IPC(8): A61K36/185G06F17/50A61P39/06A61K127/00
Inventor 邸欣马元旦刘有平王鑫
Owner SHENYANG PHARMA UNIVERSITY
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