Method for processing fungal contamination in cordyceps militaris culture process
A treatment method, the technology of Cordyceps militaris, applied in botany equipment and methods, horticulture, application, etc., can solve the problems affecting the growth of fruiting bodies, fungal pollution, etc., and achieve the effect of simple operation, low cost, and wide sources
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Embodiment 1
[0021] The cultivation of embodiment 1 Cordyceps militaris
[0022] 1. Time: April 2012:
[0023] 2. Materials:
[0024] (1) Strain: Cordyceps militaris strain with strain number HX-64 (purchased from the Biological Center of Beijing Academy of Agriculture and Forestry Sciences) was used.
[0025] (2) Strain solid medium: PDA slant.
[0026] (3) Culture medium for liquid shake flasks: 250 grams of peeled potatoes (boiled juice), 8 grams of glucose, 2.5 grams of peptone, 0.6 grams of potassium dihydrogen phosphate, 1.2 grams of magnesium sulfate, 1000 ml of water, natural pH.
[0027] (4) Nutrient solution for fruiting body production: 2.2% of soybeans, 1.5% of glucose, 4.5% of milk powder, 0.8% of potassium dihydrogen phosphate, 0.06% of magnesium sulfate, vitamin B1 15mg / 1000ml to make a nutrient solution, adjust the pH at 5.6~ 7.2.
[0028] (5) Conventional cultivation container: 12cm high, 8cm in diameter, 30g of rice, 45ml of nutrient solution.
[0029] 3. Preparation...
Embodiment 2
[0047] The dilute hydrochloric acid solution that adopts mass percent concentration to be 5% is as fungicide, and all the other are the same
[0048] Example 1.
Embodiment 3
[0050] Adopt the dilute sulfuric acid solution that mass percent concentration is 3% as fungicide, all the other are with embodiment 1.
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Abstract
Description
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