Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting angiotensin I invertase inhibitor from camellia sinensis fruit peel

An angiotensin and tea peel technology, which is applied in the field of extracting angiotensin Ⅰ converting enzyme inhibitors from tea peels, achieves a significant inhibitory effect and improves economic benefits

Active Publication Date: 2014-02-05
杭州万融茶界科技有限公司
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report or patent on extracting angiotensin Ⅰ converting enzyme inhibitor from tea peel

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting angiotensin I invertase inhibitor from camellia sinensis fruit peel
  • Method for extracting angiotensin I invertase inhibitor from camellia sinensis fruit peel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Step A: Weigh 20 g of the dried and pulverized tea peel, take the tea peel and acetone as a ratio of 1 g: 10 ml, add acetone to the tea peel, soak for 4 hours and then centrifuge to obtain the tea peel. The centrifugation condition is: 5000 rpm minutes, centrifuged for 10 minutes;

[0029] Step B: Add a buffer solution with a pH of 2 to the acetone-soaked tea peel obtained in step A at a ratio of 1 g: 15 ml to the tea peel and buffer solution, and perform ultrasonic extraction at a frequency of 200 W for 2 hours and then centrifuge to obtain the extracted solution; the buffer is preferably disodium hydrogen phosphate-citric acid buffer, and the centrifugation condition is: 5000 rpm, centrifuged for 10 minutes;

[0030] Step C: adding chloroform to the extract obtained in step B at a volume ratio of 1:1 between the extract obtained in step B and chloroform to obtain a raffinate;

[0031] Step D: Concentrating the raffinate obtained in step C and then freeze-drying to ob...

Embodiment 2

[0035] Step A: Weigh 20 g of the dried and pulverized tea peel, take the tea peel and acetone as the ratio of 1g:15ml, add acetone to the tea peel, soak for 3.5 hours and then centrifuge to obtain the tea peel. The centrifugation condition is: 5000 rpm minutes, centrifuged for 10 minutes;

[0036] Step B: Add a buffer solution with a pH of 2.5 to the acetone-soaked tea peel obtained in step A at a ratio of 1g:20ml to the tea peel and buffer solution, and perform ultrasonic extraction at a frequency of 250W for 1.5 hours and then centrifuge to obtain the extracted solution; the disodium hydrogen phosphate-citric acid buffer solution, the centrifugation condition is: 5000 rpm, centrifuged for 10 minutes;

[0037] Step C: adding chloroform to the extract obtained in step B at a volume ratio of 2:1 between the extract obtained in step B and chloroform to obtain a raffinate;

[0038] Step D: Concentrating the raffinate obtained in step C and then freeze-drying to obtain the primar...

Embodiment 3

[0042] Step A: Weigh 20 g of the dried and pulverized tea peel, take the tea peel and acetone as the ratio of 1g:20ml, add acetone to the tea peel, soak for 3 hours and then centrifuge to obtain the tea peel. The centrifugation condition is: 5000 rpm minutes, centrifuged for 10 minutes;

[0043] Step B: Add a buffer solution with a pH of 3 to the acetone-soaked tea peel obtained in step A at a ratio of 1 g: 25 ml to the tea peel and buffer solution, and perform ultrasonic extraction at a frequency of 300 W for 1 hour and then centrifuge to obtain the extracted solution; the buffer is preferably disodium hydrogen phosphate-citric acid buffer, and the centrifugation condition is: 5000 rpm, centrifuged for 10 minutes;

[0044] Step C: adding chloroform to the extract obtained in step B at a volume ratio of 3:1 between the extract obtained in step B and chloroform, and extracting to obtain a raffinate;

[0045]Step D: Concentrating the raffinate obtained in step C and then freeze...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for extracting an angiotensin I invertase inhibitor, and aims at providing a method for extracting the angiotensin I invertase inhibitor from camellia sinensis fruit peel. The method comprises the following steps: adding acetone into crushed dry camellia sinensis fruit peel for immersing, centrifuging to obtain camellia sinensis fruit peel; adding a buffer and performing ultrasonic extraction, then centrifuging to obtain an extracting solution; adding chloroform into the extracting solution, extracting to obtain raffinate; concentrating the raffinate, performing freeze drying to obtain a primer extractive; adding the primer extractive into an ethanol aqueous solution, centrifuging to obtain supernatant; and finally concentrating the supernatant and performing freeze drying to obtain the angiotensin I invertase inhibitor. By employing ultrasonic extraction and polarity screening, the natural angiotensin I invertase inhibitor is extracted from camellia sinensis fruit peel; and the angiotensin I invertase inhibitor has substantial inhibiting effect on angiotensin I invertase, and the method is capable of effectively improving economical benefit of camellia sinensis fruit peel resource.

Description

technical field [0001] The invention relates to a method for extracting angiotensin I-converting enzyme inhibitors, in particular to a method for extracting angiotensin I-converting enzyme inhibitors from tea peel. Background technique [0002] Hypertension is the most common cardiovascular disease characterized by elevated arterial systolic or diastolic blood pressure. It often causes complications in the heart, brain, kidneys, eyes, etc. It is one of the five common diseases in the world today. According to statistics in 2009, at least 1 billion people in the world have high blood pressure, and it is estimated that by 2025, the number of people with high blood pressure will increase to 1.56 billion, seriously threatening human health. Angiotensin I-converting enzyme (Agiotensin I-converting enzyme, ACE) is a zinc-containing protease bound to the cell membrane. It increases blood pressure in the renin-angiotensin-aldosterone system through the following two pathways: On the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/82A61P9/12A61K131/00
Inventor 徐平王岳飞
Owner 杭州万融茶界科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com