Method for directly planting dendrobium fimbriatum tissue culture seedlings in field

Abstract

The invention discloses a method for directly planting dendrobium fimbriatum tissue culture seedlings in a field. According to the method, culture media are optimized, and after seedlings are obtained by using the optimized culture media through tissue culture, the seedlings are planted in the specific environment and by adopting a unique matrix. By adopting the method, the dendrobium fimbriatum tissue culture seedlings are directly planted in the field, and acclimation is needless, as a result, the culturing cost of the seedlings is reduced, and transplantation survival rate and seedling obtaining rate of the seedlings are remarkably improved.

Description

technical field [0001] The invention relates to the technical field of agriculture, in particular to a direct field cultivation method for tissue cultured seedlings of dendrobium fringe. Background technique [0002] my country's traditional precious Chinese medicinal materials, Guizhou authentic Chinese medicinal materials Dendrobium fringe ( Dendrobium fimbriatum ) Fresh or dried stems, used for yin deficiency, dry mouth, polydipsia, retching due to lack of food, deficiency and heat after illness, and blurred vision ("Chinese Pharmacopoeia" 2010). Dendrobium fringe is a perennial epiphyte orchid, mainly distributed in Guizhou, Guangxi, Yunnan and other places. It is suitable for epiphyton on the surface of limestone in karst mountainous areas. Southwest Guizhou is one of the best suitable areas; Dendrobium dendrobii can be used as one of the best herb species for ecological restoration of karst rocky desertification, which can make the original barren rocky desertification...

AI Technical Summary

Problems solved by technology

[0003] At present, the "bottleneck problem" of artificial cultivation of Dendrobium fringe is that the supply of seedlings is seriously insufficient. At present, there are two main methods for providing seedlings. One is the industrial production technology of tissue cultured seedlings. This technology is relatively mature. The tissue cultured seedlings reported so far The survival rate of transplanting directly out of the bottle to the wild environment for cultivation is extremely low, and it takes more than one year to domesticate the seedlings in the greenhouse. Obtaining domesticated seedlings and then transplanting them in the field can increase the survival rate, but the domestication period of greenhouse seedlings is long and the cost is high. , because the market price of fresh Dendrobium fringe is low, and the cost of using tissue culture seedlings in greenhouse facilities is much higher than its output value, it is difficult to use and promote large-scale production

Another method is to propagate seedlings by stem cuttings. This method is simple in technology and has a high survival rate of transplanted seedlings, but this method has low reproduction coefficient (the seedling yield is only about 50%), waste of raw materials, etc. Disadvantages, so this method of reproduction is not worth advocating