Microbial leavening agent of cottonseed meal as well as preparation method thereof
A technology of microbial starter and cotton meal, applied in the field of cotton meal microbial starter and its preparation, to achieve the effects of ensuring product quality, improving efficiency and reducing costs
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Embodiment 1
[0053] Preparation of cottonseed meal microbial starter.
[0054] In this example, two strains of Saccharomyces cerevisiae with preservation number ACCC No: 20065 and Lactobacillus plantarum with preservation number ACCC No: 11118 were selected for breeding and seed production. The steps are as follows:
[0055] Step 1: Cultivate Saccharomyces cerevisiae on potato medium, subculture Lactobacillus plantarum on MRS agar medium, observe the growth rate of the strains, cultivate Saccharomyces cerevisiae colonies with a diameter ≥ 2mm, and plant milk colonies with a diameter ≥ 5mm after culturing at 30°C for 36 hours. The bacterial colony strains were stored at 4°C; the obtained Saccharomyces cerevisiae strains were inoculated in potato medium, and the Lactobacillus plantarum strains were inoculated in MRS medium, and the two strains were shaken at a constant temperature with a rotation speed of 250r / min. Cultivate in the bed at 30°C for 24 hours, take samples, and use the plate co...
Embodiment 2
[0064] Preparation of cottonseed meal microbial starter.
[0065] In this example, two strains of Saccharomyces cerevisiae with preservation number ACCC No: 20065 and Lactobacillus plantarum with preservation number ACCC No: 11118 were selected for breeding and seed production. The steps are as follows:
[0066] Step 1: Cultivate Saccharomyces cerevisiae on potato medium, subculture Lactobacillus plantarum on MRS agar medium, observe the growth rate of the strains, and cultivate Saccharomyces cerevisiae colonies with a diameter greater than 2mm and plant milk colonies with a diameter greater than 5mm after culturing at 30°C for 40 hours. The bacterial colony strains were stored at 4°C; the obtained Saccharomyces cerevisiae strains were inoculated in potato medium, and the Lactobacillus plantarum strains were inoculated in MRS medium, and the two strains were shaken at a constant temperature with a rotation speed of 250r / min. Cultivate in the bed at 30°C for 24 hours, take samp...
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