Method for extracting succinic acid from succinic acid fermentation liquor by using electrodialysis method

Abstract

The invention discloses a method for extracting succinic acid from succinic acid fermentation liquor by using an electrodialysis method, and belongs to the technical field of biochemical industry. According to the method, the succinic acid is extracted by using the steps: the ordinary succinic acid fermentation liquor is taken as a raw material, deproteinization and decoloration are carried out through thallus separation and electrodialysis, and then denatrium, concentration, crystal separation, filtration and drying are carried out in a bipolar membrane electrodialysis mode. The light transmittance of the fermentation liquor after passing through an electrodialysis device is improved to be 95% from 15%, the decoloration effect is equivalent to that of active carbon, the extraction rate is about 97-98%, and the yield of decoloration in an absorption mode is only 80-85%. Due to adoption of the method, the decoloration by using the active carbon is changed, the absorption of the active carbon on the succinic acid is avoided, and the loss of the succinic acid after the decoloration is avoided, so that the yield is increased; molecular succinic acid is obtained, and no hydrochloric acid or sulfuric acid is required to be used for adjusting the pH value of a system, so that the purification on the succinic acid can be easily carried out; no organic solvents are used, so that residue of the organic solvents is avoided.

Description

technical field [0001] The invention relates to a method for extracting succinic acid from a succinic acid fermentation broth prepared by a fermentation method using electrodialysis, especially for deproteinization and decolorization using anion dialysis and electrodialysis, and a mediation system for bipolar membrane electrodialysis The pH value process belongs to the technical field of biochemical industry. Background technique [0002] Succinic acid is an important organic chemical raw material and intermediate, mainly used in the pharmaceutical industry, food industry, chemical industry, etc., and is a derivative platform of C4 compounds. Traditional succinic acid is obtained through petrochemical and coal chemical industries. In recent years, researchers at home and abroad have found that they can use bacterial fermentation to convert long-chain plant fibers into succinic acid, which can be achieved at low cost. Get succinic acid. [0003] There are a vari...

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Problems solved by technology

[0007] In the above-mentioned patent, there are some problems as follows in the method of extracting succinic acid from the fermentation liquid, which is not conducive to the promotion of large-scale production of succinic acid prepared by fermentation

[0008] 1. In the disclosed method, resin or activated carbon is used for decolorization, but in the decolorization process, due to the adsorption of succinic acid by resin and activated carbon, the loss of succinic acid in the decolorized aqueous solution is too large, which reduces the extraction rate

[0009] 2. In order to obtain molecular succinic acid, it is necessary to use hydrochloric acid or sulfuric acid to adjust the pH value of the system, so chloride ions or sulfate ions are introduced into the system, which increases the difficulty of product purification

[0010] 3. The use of organic solvents in the extraction process may cause organic solvent residues in the product, which affects the use of succinic acid in the food field

In the author's research, it is found that the electrodialysis device can effectively separate the succinate ion from the protein and pigment in the fermentation broth. It is found in the research that protein is a high molecular compound and cannot be ionized by itself, while some pigments are in an ionic state, but Due to the large molecular weight, complex molecular structure, and large space volume, it is difficult to pass through the ion exchange membrane.

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