Preparation method for nanoparticles with small particle size and nanoparticle medicine carrier
A nanoparticle and drug technology, which is applied in the preparation of small-sized nanoparticles and nanoparticle drug carriers, can solve the problems of low biosafety, inability to use tumor growth and recurrence, and poor biocompatibility of nano-anticancer drugs. Achieve the effects of simple preparation method, wide application and strong tumor penetration
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[0019] Specifically, the preparation method of the present invention mainly includes four steps: preparing a solution, forming a phospholipid film, self-assembling the nano-precipitate, and collecting by ultrafiltration. It should be understood that although these steps are described in this order below, the specific order of these steps is not limited thereto, for example, it is not necessary to prepare all the solutions first, and it is also possible to prepare only the phospholipid film formation and nanoprecipitation self-assembly steps to be used in this step. solution.
[0020] Solution preparation steps: Dissolve PLGA in acetonitrile, although the concentration has little effect on the preparation of nanoparticles, usually, the concentration of 1-10mg / ml can be used; dissolve lecithin and DSPE-PEG in ethanol respectively In the aqueous solution, a lecithin solution and a DSPE-PEG solution are formed. Because the solubility of phospholipids in water is low, aqueous ethan...
Embodiment 1
[0031] Preparation solution: acetonitrile solution of PLGA, the concentration is 2.5mg / ml; solution of lecithin and DSPE-PEG in 4% ethanol aqueous solution, the concentration is 1mg / ml.
[0032] Phospholipid film formation: add 800 μl of lecithin solution and DSPE-PEG solution to 2ml of 4% ethanol solution, wherein the mass ratio of lecithin: DSPE-PEG is 1:1, and spin dry after ultrasonic mixing to form a uniform phospholipid film.
[0033] Nano-precipitation self-assembly: Mix 3.2ml of PLGA solution into 4ml of 4% ethanol aqueous solution, shake and mix, add dropwise to the film-forming container, heat to 65°C and keep stirring during the dropwise addition. The mass ratio of PLGA to total phospholipids is 5:1. After completion of the dropwise addition, keep stirring in a water bath at 65° C. for 2 hours.
[0034] Ultrafiltration collection: Collect by centrifugation with a 10kDa ultrafiltration tube to obtain nanoparticles with small particle size.
[0035] The average par...
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