Method for identifying DNA barcodes of three gentiana macrophylla medicinal materials in pharmacopeia
A technology of barcode and Gentiana, applied in the field of DNA barcode identification
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[0020] 1 Experimental equipment and reagents
[0021] 1.1 Experimental Instruments
[0022] DDY-6C electrophoresis instrument (Beijing Liuyi Instrument Factory), T196 PCR amplification instrument (German Biometra), GeCDOCXR gel imaging system (Bio-RAD, USA), HANGPINGJA3003N electronic analytical balance, KM-15200 KUBOTA centrifuge, eppendorfCentrrfuge 5417R ultra-fast refrigerated centrifuge (Eppendorf, Germany), Guohua HH-60 digital display constant temperature stirring circulating water tank, BCD-2181E refrigerator (Royalstar), Panasonic NM-S553MF microwave oven (Shanghai Panasonic Microwave Oven Co., Ltd.), KQ-250DB CNC ultrasonic wave Cleaner (Kunshan Ultrasonic Instrument Co., Ltd.), SAXXYO MDF-382E-80°C ultra-low temperature refrigerator (Japan), Aquapro AYJ1-0501-U ultrapure water system (Yiyang Enterprise Development Co., Ltd.), LQP-B- 4 Ice maker (Liuquan Anke), PHOTOA drying oven (Shanghai Yiheng Technology Co., Ltd.), SK-1 fast mixer (Guohua), TopPette manual singl...
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