PCR detection kit for goat tuberculosis

A detection kit and tuberculosis technology, applied in the field of kits, can solve the problems of low detection rate, cumbersome and time-consuming, poor sensitivity, etc., and achieve the effects of high specificity, simple operation and rapid detection

Inactive Publication Date: 2014-01-15
GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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  • Abstract
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  • Application Information

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Problems solved by technology

The traditional detection method of Mycobacterium bovis is mainly bacteriological examination, but due to cumbersome time-consuming, low detection rate and poor sensitivity, it can no longer meet the needs of current epidemic quarantine
Separation and culture require a long period, usually 1 to 2 months
However, the traditional tuberculin intradermal allergy (PPD) detection method has been reported to have non-specific reactions.

Method used

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  • PCR detection kit for goat tuberculosis
  • PCR detection kit for goat tuberculosis
  • PCR detection kit for goat tuberculosis

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Embodiment Construction

[0057] Embodiments of the present invention: goat tuberculosis PCR detection kit, which includes 250 μL of PCR premix buffer, 150 μL of ultrapure water, 50 μL of Marker DL2000, 40 μL of upper primers with a concentration of 10 μmol / L and 40 μL of lower primers with a concentration of 10 μmol / L Primers, 20 μL negative control and 20 μL positive control;; the sequence of the upstream primer is: 5'-ATCAGCGACTACCTGGCCGAAG-3'; the sequence of the downstream primer is: 5'-GTGGCGTGCCGTTCTCGTCG-3'; the negative control is ultrapure water; the positive control standard goat PCR product of Mycobacterium tuberculosis; PCR premix buffer consists of 3 mmol / ul MgCl 2 , 500pmol / ul dNTP 2.0μL, 25mmol / μL Tris-HCl and 0.2μL Taq enzyme, its pH value is pH8.3.

[0058] SEQUENC E LISTING

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Abstract

The invention discloses a PCR detection kit for goat tuberculosis. The embodiment of the invention is a PCR detection kit for goat tuberculosis, the PCR detection kit comprises 250 [mu]L of PCR premix buffer, 150 [mu]L of ultrapure water, 50 [mu]L of Marker DL2000, 40 [mu]L of upper primer with a concentration of 10 [mu]mol/L, 40 [mu]L of lower primer with a concentration of 10 [mu]mol/L, 20 [mu]L of negative reference, and 20 [mu]L of positive reference. The primer is designed according to specific gene sections of mycobacterium bovis, and a PCR detection method for diagnosing goat tuberculosis is established by utilizing the primer. The primer is applied to the PCR rapid detection kit, so that the kit can rapidly detect the goat tuberculosis, and has a very good specificity and sensitivity. The detection result of the PCR detection kit is accurate, and the PCR detection kit has the advantages of rapidness, sensitivity, simple operation, and good using effect.

Description

technical field [0001] The invention relates to a kit, in particular to a PCR detection kit for goat tuberculosis. Background technique [0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis in humans, livestock and poultry. Goat tuberculosis is a zoonotic chronic infectious disease caused by Mycobacterium bovis (M.bovis), which is listed as a B-type animal disease by the International Office of Epizootics (OIE). development and human health. The traditional detection method of Mycobacterium bovis is mainly bacteriological examination, but due to cumbersome and time-consuming, low detection rate and poor sensitivity, it can no longer meet the needs of current epidemic quarantine. Separation and culture requires a long period, usually 1 to 2 months. However, the traditional tuberculin intradermal allergy (PPD) detection method has been reported to have non-specific reactions. Contents of the invention [0003] The purpose of the prese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04
CPCC12Q1/04C12Q1/686C12Q2531/113
Inventor 冉懋韬余波谭诗文王凡艾玉萍
Owner GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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