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A strain of Stenotrophomonas and its application in the control of cyanobacterial blooms

A technology of oligotrophomonas and cyanobacteria bloom, which is applied in the field of oligotrophomonas Lzh-7 and its application in the control of cyanobacteria bloom, can solve the problems of secondary pollution and limited processing capacity, and achieve good results The effect of lethal effect, short preparation cycle and simple preparation method

Inactive Publication Date: 2015-09-09
SHANGHAI JIAOTONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of the limited processing capacity of physical and chemical methods in the prior art, and the defects of easily causing secondary pollution, the technical problem to be solved in the present invention is to find efficient algae-dissolving bacteria and to separate and enrich the highly efficient algae-dissolving active substances produced by the metabolism of algae-dissolving bacteria , for safe and efficient control of cyanobacterial blooms

Method used

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  • A strain of Stenotrophomonas and its application in the control of cyanobacterial blooms
  • A strain of Stenotrophomonas and its application in the control of cyanobacterial blooms
  • A strain of Stenotrophomonas and its application in the control of cyanobacterial blooms

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Embodiment 1

[0036] Example 1 Screening of algicidal bacteria

[0037] 10 mL of natural water samples collected from the waters of Meiliang Bay of Taihu Lake were added to 90 mL of logarithmic phase of Microcystis aeruginosa 9110 algal solution. Two days later, the yellow algal solution was taken and coated with beef extract peptone medium agar plate by gradient dilution method, and cultured at 28°C. 24h, take the plate with moderate colony density, and select different strains according to the different colony morphology.

[0038] The screened strains were inoculated into 10 mL of beef extract peptone medium, cultured at 28°C and 200 rpm for 24 h, and 10 mL of the cultured bacterial solution was added to 90 mL of log phase Microcystis aeruginosa algal solution. In addition, 10 mL of sterilized beef extract peptone medium was also added to 90 mL of algae liquid as a control. The algae-dissolving efficiency of all experimental groups and control groups was calculated after culturing in a l...

Embodiment 2

[0042] Embodiment 2 Identification of oligotrophomonas Lzh-7 strain

[0043] The morphological observation, staining, physiological and biochemical reactions, 16srRNA gene sequence analysis and other methods were used to identify Lzh-7 with strong algae-dissolving effect. , arranged singly or in pairs, one end of a commensal fungus, with motility. The optimum growth temperature is 35 ℃, obligate aerobic bacteria. Colonies were medium in size, round, yellow or tan opaque on beef extract peptone medium plates. Negative for oxidase, positive for DNase, positive for lysine decarboxylase test, slow oxidative decomposition of glucose, rapid oxidative decomposition of maltose, liquefaction of gelatin, negative for nitrogen production from nitrate. After 16srRNA gene sequence analysis and homology comparison, it was found that it has 99% homology with a strain of Stenotrophomonas in GenBank, so it was identified as Stenotrophomonas and named as Stegotrophomonas. Lzh-7. The strain ...

Embodiment 3

[0044] Embodiment 3 The preparation method of oligotrophomonas Lzh-7 fermentation broth and its ethyl acetate extract

[0045] Stenotrophomonas Lzh-7 was inoculated into sterilized beef extract peptone medium at 1% inoculum, and cultured at 28°C on a 200 rpm shaker for 48 hours to obtain a bacterial-containing fermentation broth of Monotrophomonas Lzh-7. Add ethyl acetate into the fermentation broth at a ratio of 1:1, put it into a shaker to shake for 24 hours, and separate the upper layer solution, that is, the ethyl acetate extract. The ethyl acetate was evaporated to dryness, dissolved in water, and filtered through a microporous membrane with a 0.22 μm pore size for further purification of metabolites.

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Abstract

The invention discloses a Stenotrophomonas sp. strain and application thereof in controlling cyanobacterial blooms. A strain of Stenotrophomonas sp. Lzh-7 (CGMCC No.6548) having significant algae-lysing activity is isolated from Taihu water body. The fact is identified that the effective algae-lysing component hydroquinone is isolated and purified from the metabolites of the strain, wherein the median lethal dose (LD50) of hydroquinone to Microcystis aeruginosa 9110 is 3.9 Mug / mL and the median lethal dose (LD50) of hydroquinone to Synechococcus BN60 is 6.7 Mug / mL. The Stenotrophomonas sp. strain can be applied to the research, development and production of novel biological algaecides and eventually applied to the control of lake cyanobacterial blooms.

Description

technical field [0001] The invention relates to the field of environmental microorganisms, in particular to a strain of Stenotrophomonas sp. Lzh-7 with algal lytic activity and its application in the control of cyanobacterial blooms. Background technique [0002] In recent years, the outbreak of cyanobacteria blooms caused by eutrophication of lakes has increasingly polluted the lake water system. Freshwater waters such as Taihu Lake, Chaohu Lake, and Dianchi Lake have been seriously affected, and the environmental and economic problems caused by them have increasingly attracted people's attention. Therefore, it is very necessary to explore effective ways to control cyanobacterial biomass and inhibit the occurrence of cyanobacterial blooms. [0003] The control technologies of cyanobacterial blooms can be classified into physical methods, chemical methods and biological methods. Physical methods such as mechanical algae removal, electromagnetic field algae removal, etc. can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F1/00C02F3/34C12R1/01
Inventor 杨虹李正华潘建良柳向龙
Owner SHANGHAI JIAOTONG UNIV
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