A high-yielding cellulase strain and method for producing neutral cellulase by fermentation

Abstract

The invention provides a Trichodema reesei QZ‑5 strain of Trichodema reesei with high cellulase production, which has been preserved in the China Center for Type Culture Collection, with a preservation number of CCTCC NO: M2012204 and a preservation date of June 2012 5th. At the same time, a method of using this bacterium as the starting strain, cultivating seeds, using corn steep liquor, ammonium sulfate, etc. as nitrogen sources, and using glucose, bran, etc. as carbon sources to ferment and produce neutral cellulase was also invented. The activity can reach more than 5000IU / L, and the neutral cellulase produced can be widely used in textile, papermaking, feed, food and other fields, belonging to the field of biotechnology.

Description

technical field [0001] The present invention utilizes a high-yield cellulase strain (Trichodema reesei QZ-5) and utilizes the strain to produce neutral cellulase through seed cultivation, corn steep liquor, ammonium sulfate, etc. as nitrogen sources, and glucose, bran, etc. as carbon sources. The cellulase method, the prepared enzyme can be applied to the fields of textile, papermaking, feed, food and the like, and belongs to the field of biotechnology. Background technique [0002] Cellulase is a kind of multi-component complex enzyme system that can degrade cellulose into glucose, which can be widely used in energy, textile, papermaking, feed, food and other fields. In recent years, with the increasing shortage of global resources and energy, Cellulase is the main enzyme system that uses the most widely distributed, most abundant, and cheapest cellulose in nature as a renewable resource, so the research on the production and preparation of cellulase has become a topic of m...

AI Technical Summary

Problems solved by technology

Although my country has become the fourth country in the world that can produce cellulase after the United States, Japan, and Denmark, the production technology of neutral cellulase in my country is still relatively backward compared with developed countries, with low enzyme activity, high cost and small scale. , the root cause is that no wild strains with high enzyme activity have been screened or the technology of genetically modified engineered bacteria is still in the development stage

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