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High-efficient denitrifying flocculant-producing bacterial strain and application thereof

A denitrification and high-efficiency technology, applied in the direction of bacteria, water/sludge/sewage treatment, water pollutants, etc., can solve problems such as long start-up time, slow growth of nitrifying bacteria, difficult biomass, and increased operating costs

Inactive Publication Date: 2014-01-08
CHENGDU UNIV OF INFORMATION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These traditional denitrification processes have played a certain role in wastewater treatment, but there are some defects: ①The process flow is complicated, and the growth rate of nitrifying bacteria is slow to maintain a high biomass; ②The nitrification process needs to be in the presence of oxygen. Complete, so the consumption of oxygen is large; ③The nitrification process also produces a large amount of acid, which needs to be neutralized by adding alkali, which increases the operating cost
[0004] In recent years, there have been reports at home and abroad about some aerobic denitrifying bacteria with nitrifying properties at the same time, which can convert NH 4 + ~N is directly converted into gaseous nitrogen and discharged directly from the water body. This kind of bacteria has high utilization value and helps to solve the traditional biological denitrification process. Disadvantages such as middle progress

Method used

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  • High-efficient denitrifying flocculant-producing bacterial strain and application thereof
  • High-efficient denitrifying flocculant-producing bacterial strain and application thereof
  • High-efficient denitrifying flocculant-producing bacterial strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Example 1: Acquisition of the high-efficiency denitrification and flocculation-producing strain

[0091] Take an appropriate amount of sludge in the Sequencing Batch Bioreactor (SBR) system, inoculate it in a heterotrophic nitrification medium for culture, and then transfer it to an aerobic denitrification medium for denitrification culture after 1 to 2 days. Take 0.5-1mL of bacterial suspension, and carry out gradient dilution with sterilized distilled water, and take 10μL for a dilution factor of 10 3 ~10 10 2 times the bacterial suspension was plated on the BTB isolation medium, and after 2-5 days of culture, the colony that caused the blue halo to appear in the surrounding medium was picked as the primary screening bacteria; Repeat the streaking to obtain pure single strains, and observe them morphologically, as figure 1 shown. Take the 18-24h bacterial culture solution and fill it in the basic medium containing glycerol at a volume ratio of 1:2, mark it well, an...

Embodiment 2

[0092] Example 2: Denitrification and flocculation performance of the high-efficiency denitrification and flocculation-producing strain

[0093] 1) Nitrogen removal performance

[0094] (1) Heterooxygen nitrification ability: Take a single colony of Acinetobacter sp.TN-14 and inoculate it into a heterotrophic nitrification medium at a volume ratio of 1-5%, at 30°C, 160- Shake and cultivate at 220rpm for 48 hours, and measure the OD of bacterial growth in the culture medium at intervals of 6 hours 600 , ammonia nitrogen, nitrate nitrogen, nitrite nitrogen and TN concentration changes. Such as figure 2 As shown in the test calculation, within 24 hours, the removal rate of the strain to ammonia nitrogen and total nitrogen reached 97.13% and 93.53%, respectively, and there was basically no accumulation of nitrate nitrogen and nitrite nitrogen.

[0095] (2) Aerobic denitrification ability: Take a single colony of Acinetobacter sp.TN-14 and inoculate it with NO 3 - -N and NO ...

Embodiment 3

[0100] Example 3: The specific operation of the high-efficiency nitrogen-removing floc-producing strain applied to the treatment of domestic sewage

[0101] Pick a single colony of Acinetobacter sp.TN-14, activate it in a heterotrophic nitrification medium for 20-24 hours, then take its activation solution, and inoculate it into In the sewage to be treated, cultivate at 30°C and 160-220rpm for 12-24h. The sewage to be treated [COD]=250~400mg / L; [NH 4 + ~N]=25~55mg / L, [TN]=30~60mg / L, [TP]=3.0~8.0mg / L.

[0102] The change of COD concentration in sewage before and after treatment was measured. After testing and calculation, the removal rates of COD, ammonia nitrogen and TN in actual domestic sewage reached 76.10%, 96.53% and 96.65% respectively within 12 hours.

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Abstract

The invention relates to a high-efficient denitrifying flocculant-producing bacterial strain with a preservation name of acinetobacter TN-14 (Acinetobacter sp. TN-14), which was preserved in China Center for Type Culture Collection at June 5, 2013 with a preservation number of CCTCC No. M2013247. The invention also relates to an application of the high-efficient denitrifying flocculant-producing bacterial strain. The high-efficient denitrifying flocculant-producing bacterial strain has good capability of heterotrophic nitrification, aerobic denitrification, and removal of COD, ammonia nitrogen and TN in real life sewage, and fermentation media or substitutive media are used for fermentation and flocculant production. The bacterial strain has extremely high removal rate for ammonia nitrogen and total nitrogen in systems of heterotrophic nitrification and denitrification; extremely high removal rate for COD, ammonia nitrogen, TN and TP in life sewage and pig farm waste water; and extremely high flocculation rate when the bacterial strain fermentation liquor is added into kaolin suspending liquid.

Description

technical field [0001] The invention relates to a high-efficiency denitrification and flocculation-producing bacterial strain, in particular to a new Acinetobacter sp. TN-14 strain. Background technique [0002] With the development of industrial and agricultural production and the improvement of people's living standards, the discharge of nitrogen-containing compounds has increased sharply, and has become a major source of environmental pollution, attracting attention from all walks of life. Ammonia nitrogen is the main form of nitrogen in the aqueous environment, and it is an important pollutant that causes water eutrophication and environmental pollution. Ammonia nitrogen in surface water has gradually become the most important pollution item, even surpassing chemical oxygen demand to become the primary indicator affecting the environmental quality of surface water. For water bodies, the "National Environmental Protection "Twelfth Five-Year Plan" will continue to reduce ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12P1/04C02F101/38C02F103/20
Inventor 信欣姚力周迎芹鲁磊冷璐
Owner CHENGDU UNIV OF INFORMATION TECH
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